Persistent Increases of PKMζ in Sensorimotor Cortex Maintain Procedural Long-Term Memory Storage

IScience
Peng Penny GaoJoseph Thachil Francis

Abstract

Procedural motor learning and memory are accompanied by changes in synaptic plasticity, neural dynamics, and synaptogenesis. Missing is information on the spatiotemporal dynamics of the molecular machinery maintaining these changes. Here we examine whether persistent increases in PKMζ, an atypical protein kinase C (PKC) isoform, store long-term memory for a reaching task in rat sensorimotor cortex that could reveal the sites of procedural memory storage. Specifically, perturbing PKMζ synthesis (via antisense oligodeoxynucleotides) and blocking atypical PKC activity (via zeta inhibitory peptide [ZIP]) in S1/M1 disrupts and erases long-term motor memory maintenance, indicating atypical PKCs and specifically PKMζ store consolidated long-term procedural memories. Immunostaining reveals that PKMζ increases in S1/M1 layers II/III and V as performance improved to an asymptote. After storage for 1 month without reinforcement, the increase in M1 layer V persists without decrement. Thus, the persistent increases in PKMζ that store long-term procedural memory are localized to the descending output layer of the primary motor cortex.

Citations

Oct 22, 2019·Frontiers in Integrative Neuroscience·Dong LiClaus C Hilgetag
Mar 7, 2020·Scientific Reports·Thiago Rodrigues da SilvaCristina A Stern
Jun 7, 2020·Journal of Molecular Neuroscience : MN·Yogesh Kumar Dhuriya, Divakar Sharma
Mar 15, 2019·Scientific Reports·Anastasia A BorodinovaPavel M Balaban
Dec 30, 2018·Molecular Brain·Todd Charlton Sacktor, André Antonio Fenton
Feb 9, 2021·Reviews in the Neurosciences·Hamish Patel, Reza Zamani
Feb 5, 2021·The European Journal of Neuroscience·Changchi HsiehTodd Charlton Sacktor
Apr 4, 2020·Current Biology : CB·Peter S B Finnie, Karim Nader
Aug 7, 2021·Scientific Reports·Md Moin Uddin Atique, Joseph Thachil Francis

❮ Previous
Next ❯

Methods Mentioned

BETA
antisense oligodeoxynucleotides

Related Concepts

Related Feeds

Barrel cortex

Here is the latest research on barrel cortex, a region of somatosensory and motor corticies in the brain, which are used by animals that rely on whiskers for world exploration.

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.