Persistent propagation of variant Creutzfeldt-Jakob disease agent in murine spleen stromal cell culture with features of mesenchymal stem cells.

Journal of Virology
Sergey AkimovL Cervenakova

Abstract

The transmission of variant Creutzfeldt-Jakob disease (vCJD) through blood transfusions has created new concerns about the iatrogenic spread of transmissible spongiform encephalopathies (TSEs)/prion diseases through blood and plasma-derived products and has increased the need to develop efficient methods for detection of the agent in biologics. Here, we report the first successful generation of spleen-derived murine stromal cell cultures that persistently propagate two mouse-adapted isolates of human TSE agents, mouse-adapted vCJD, and Fukuoka 1. These new cell cultures can be used efficiently for studies of the pathogenesis of the disease, for development of diagnostics and therapeutics, and as a rapid ex vivo assay for TSE inactivation/removal procedures.

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Citations

Oct 4, 2015·The Journal of General Virology·Diego R MedianoInmaculada Martín-Burriel
May 24, 2014·Zoonoses and Public Health·D R MedianoI Martín-Burriel
Feb 18, 2009·Vox Sanguinis·J CosteUNKNOWN Subgroup on TSE
Dec 3, 2014·Virus Research·Paula Saá, Larisa Cervenakova
May 1, 2021·Animals : an Open Access Journal From MDPI·Laura García-MendívilInmaculada Martín-Burriel

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