Jan 27, 2017

Persistent stromal fibroblast activation is present in chronic tendinopathy

Arthritis Research & Therapy
Stephanie Georgina DakinAndrew Jonathan Carr

Abstract

Growing evidence supports a key role for inflammation in the onset and progression of tendinopathy. However, the effect of the inflammatory infiltrate on tendon cells is poorly understood. We investigated stromal fibroblast activation signatures in tissues and cells from patients with tendinopathy. Diseased tendons were collected from well-phenotyped patient cohorts with supraspinatus tendinopathy before and after sub-acromial decompression treatment. Healthy tendons were collected from patients undergoing shoulder stabilisation or anterior cruciate ligament repair. Stromal fibroblast activation markers including podoplanin (PDPN), CD106 (VCAM-1) and CD248 were investigated by immunostaining, flow cytometry and RT-qPCR. PDPN, CD248 and CD106 were increased in diseased compared to healthy tendon tissues. This stromal fibroblast activation signature persisted in tendon biopsies in patients at 2-4 years post treatment. PDPN, CD248 and CD106 were increased in diseased compared to healthy tendon cells. IL-1β treatment induced PDPN and CD106 but not CD248. IL-1β treatment induced NF-κB target genes in healthy cells, which gradually declined following replacement with cytokine-free medium, whilst PDPN and CD106 remained above pre-stim...Continue Reading

  • References26
  • Citations24

References

  • References26
  • Citations24

Citations

Mentioned in this Paper

Biological Markers
Establishment and Maintenance of Localization
Vcam1
Real-Time Polymerase Chain Reaction
Buffers
Tendinopathy
3,3'-Diaminobenzidine
IL1A
Flow Cytometry
Immunofluorescence Assay

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