PMID: 8952331Oct 1, 1996Paper

Personal identification using DNA polymorphism--the identification of forensic biological materials

Nihon hōigaku zasshi = The Japanese journal of legal medicine
H Shiono

Abstract

We performed personal identification by DNA polymorphism using forensic specimens. DNA was purified by the potassium iodine method instead of the phenol extraction method. When DNA is amplified by PCR, there are inhibitors of PCR such as melanin in hairs or blood and inorganic salts in bone. This problem was solved by the potassium iodine method. The genotype of the ABO blood groups could be determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in all 29 old blood-stain specimens (obtained 11 months-15 years before) and 14 fingerprint specimens on a glass plate. In sexual assaults against women, one key to identifying the suspect is ABO phenotyping or the typing of other polymorphic markers of the seminal fluid in the victim's vagina. However, ABO phenotyping is frequently unsuccessful, since mixtures of fluids cannot be separated to be subjected to conventional methods for the detection of antibody or antigen material. We therefore studied ABO blood group genotyping of sperm DNA isolated from contaminating vaginal fluid by the PCR-RFLP method. Seminal samples of genotypes OO, AO, BO and AB were experimentally mixed with vaginal fluid (OO, AO, BO and AB), and were successfully se...Continue Reading

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