Abstract
There are no reliable blood biomarkers for monitoring endometrial cancer patients in the current clinical practice. Circulating tumor DNA (ctDNA) is emerging as a promising non-invasive method to measure tumor burden, define prognosis and monitor disease status in many solid cancers. In this pilot study, we investigated if unique tumor-specific DNA junctions can be used to detect ctDNA levels in patients with endometrial cancer. Chromosomal rearrangements in primary tumors of eleven patients with high-grade or advanced stage endometrial cancer were determined by whole-genome Mate-Pair sequencing. Identified unique tumor-specific junctions were evaluated in pre- and six-week post-surgery patient plasma using individualized quantitative polymerase chain reaction (qPCR) assays. The relationship between clinicopathological features and detection of ctDNA was investigated. CtDNA was detected in 60% (6/10) of cases pre-surgery and in 27% (3/11) post-surgery. The detection of ctDNA pre-surgery was consistent with clinical indicators of aggressive disease such as advanced stage (80% - 4/5), lymphatic spread of disease (100% - 3/3), serous histology (80% - 4/5), deep myometrial invasion (100% - 3/3), lympho-vascular space invasion (75% ...Continue Reading
References
Dec 8, 2011·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Hideo TanakaDaisuke Aoki
Mar 15, 2013·The New England Journal of Medicine·Sarah-Jane DawsonNitzan Rosenfeld
Apr 9, 2013·Nature·Muhammed MurtazaNitzan Rosenfeld
Jul 9, 2013·Scientific Reports·Daichi ShigemizuTatsuhiko Tsunoda
Feb 15, 2014·Bioinformatics·Travis M DruckerGeorge Vasmatzis
May 24, 2014·Analytical and Bioanalytical Chemistry·Alison S DevonshireJim F Huggett
Jul 9, 2014·Obstetrics and Gynecology·Kathleen J YostSean C Dowdy
Aug 28, 2015·Science Translational Medicine·Isaac Garcia-MurillasNicholas C Turner
Dec 31, 2015·PloS One·Elena PereiraJohn A Martignetti
Jul 8, 2016·Science Translational Medicine·Jeanne TiePeter Gibbs
Jul 21, 2016·Scientific Reports·Faye R HarrisGeorge Vasmatzis
Oct 28, 2017·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Yuki IijimaMasao Omata
Dec 28, 2017·Bioinformatics·Athanasios GaitatzesGeorge Vasmatzis
Feb 1, 2018·Journal of Experimental & Clinical Cancer Research : CR·Enrico VizzaLucia Cicchillitti
Feb 7, 2018·Cancer Genetics·Sarah H JohnsonGeorge Vasmatzis
Feb 22, 2018·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Karin AbbinkLeon Fag Massuger
May 5, 2018·Genes, Chromosomes & Cancer·James B SmadbeckGeorge Vasmatzis
Jul 20, 2019·Scientific Reports·Naoyuki IwahashiKazuhiko Ino
Feb 27, 2020·Journal of Clinical Medicine·Carlos Casas-ArozamenaLaura Muinelo-Romay
Mar 17, 2020·Clinical Chemistry·Amanda Bortolini SilveiraMarc-Henri Stern