Perspectives for Glyco-Engineering of Recombinant Biopharmaceuticals from Microalgae.

Cells
Lorenzo BaroloMathieu Pernice

Abstract

Microalgae exhibit great potential for recombinant therapeutic protein production, due to lower production costs, immunity to human pathogens, and advanced genetic toolkits. However, a fundamental aspect to consider for recombinant biopharmaceutical production is the presence of correct post-translational modifications. Multiple recent studies focusing on glycosylation in microalgae have revealed unique species-specific patterns absent in humans. Glycosylation is particularly important for protein function and is directly responsible for recombinant biopharmaceutical immunogenicity. Therefore, it is necessary to fully characterise this key feature in microalgae before these organisms can be established as industrially relevant microbial biofactories. Here, we review the work done to date on production of recombinant biopharmaceuticals in microalgae, experimental and computational evidence for N- and O-glycosylation in diverse microalgal groups, established approaches for glyco-engineering, and perspectives for their application in microalgal systems. The insights from this review may be applied to future glyco-engineering attempts to humanize recombinant therapeutic proteins and to potentially obtain cheaper, fully functional b...Continue Reading

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Aug 28, 2020·Frontiers in Chemistry·Thapakorn JaroentomeechaiMatthew P DeLisa
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Aug 29, 2021·World Journal of Microbiology & Biotechnology·Imke de Grahl, Sigrun Reumann

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Methods Mentioned

BETA
glycosylation
transgenic
acetylation
human

Software Mentioned

BLASTp
PFAM
EMBOSS
BLAST
Basic Local Alignment Tool ( BLAST )
HMMER

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