Perspectives on and Precautions for the Uses of Electric Spectroscopic Methods in Label-free Biosensing Applications

ACS Sensors
Beatriz L GarrotePaulo R Bueno

Abstract

Label-free approaches for molecular diagnostic applications are appealing because of their inherent point-of-care advantages. Nonetheless, technical challenges impose a limit on the use of these methods as will be discussed in this paper. Electrochemical spectroscopic methods, such as impedance and impedance-derived methods, are highly effective in the development of label-free diagnostic assays, but they require careful control of the dynamics of the sensing interface. We herein report the strength and challenges of the current methodologies associated with the applications of impedance and impedance-derived methods by focusing on their principles of operation. We demonstrate that the uses of their potentialities are not based on the know-how of these methods, but on how to combine the spectroscopic features with the required chemical design for the associated sensing interfaces. Predominantly, we illustrate how to use the resistive and capacitive terms of the interface to improve its sensitivity to the target. For instance, with the proper signal amplification strategy, limitations related to target-to-receptor size ratio can be overcome. The target-to-receptor ratio is one of the difficulties that we use as an example to ill...Continue Reading

References

Apr 1, 1997·The Analyst·T WinkW P van Bennkom
Nov 5, 1997·Clinical Immunology and Immunopathology·B E Barton
Aug 5, 2003·The Journal of Clinical Investigation·Cora SchaferWilli Jahnen-Dechent
Dec 7, 2005·Biosensors & Bioelectronics·Joseph Wang
Feb 16, 2007·Physical Chemistry Chemical Physics : PCCP·Lawrence Yoon Suk Lee, R Bruce Lennox
Jan 8, 2008·Electroanalysis·Jonathan S Daniels, Nader Pourmand
Apr 17, 2008·Analytical and Bioanalytical Chemistry·F Lisdat, D Schäfer
Jun 19, 2008·Analytica Chimica Acta·Xudong FanYuze Sun
Nov 18, 2008·Biosensors & Bioelectronics·Abd-Elgawad RadiJean-Louis Marty
Apr 15, 2009·Analytical Chemistry·A BogomolovaM Aldissi
May 10, 2012·Nature Communications·T Albrecht
Mar 7, 2013·Analytical Chemistry·Xiliang LuoJason J Davis
Apr 26, 2013·Chemical Society Reviews·Xiliang Luo, Jason J Davis
Jul 31, 2013·Biosensors & Bioelectronics·Flávio C Bedatty FernandesPaulo R Bueno
Feb 25, 2014·Biosensors & Bioelectronics·Flávio C B FernandesPaulo R Bueno
Sep 12, 2014·Artificial Cells, Nanomedicine, and Biotechnology·Elif Burcu Bahadır, Mustafa Kemal Sezgintürk
Dec 10, 2014·Analytical Chemistry·Amol V PatilJason J Davis
Jan 16, 2015·Biosensors & Bioelectronics·Adriano SantosPaulo R Bueno
Nov 20, 2015·Analytical Chemistry·Flavio Cesar Bedatty FernandesJason J Davis
Sep 8, 2016·Nature Communications·Maria R LukatskayaYury Gogotsi
Sep 26, 2016·Biosensors & Bioelectronics·Juliana CecchettoPaulo R Bueno
Jun 1, 2017·Physical Chemistry Chemical Physics : PCCP·Joshua LehrPaulo R Bueno
Oct 4, 2017·Nanoscale·Paulo R BuenoJason J Davis
Oct 7, 2017·Biosensors & Bioelectronics·Adriano SantosJason J Davis
Nov 29, 2017·Biosensors & Bioelectronics·Fabiana S Felix, Lúcio Angnes
Feb 8, 2018·Analytical Chemistry·Julia PiccoliJason J Davis
May 25, 2018·Analytical Chemistry·Paulo R Bueno

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Citations

Feb 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sławomir LachSylwia Rodziewicz-Motowidło
Aug 14, 2020·Analytical Methods : Advancing Methods and Applications·Hugo José Nogueira Pedroza Dias MelloMarcelo Mulato
Oct 28, 2020·Nature Protocols·Beatriz L GarrotePaulo R Bueno

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