Perturbation of dynamin II with an amphiphysin SH3 domain increases GLUT4 glucose transporters at the plasma membrane in 3T3-L1 adipocytes. Dynamin II participates in GLUT4 endocytosis.

The Journal of Biological Chemistry
A VolchukA Klip

Abstract

The GLUT4 glucose transporter continuously recycles between the cell surface and an endosomal compartment in adipocytes. Insulin decreases the rate of GLUT4 endocytosis in addition to increasing its exocytosis. Endocytosis of the transporter is thought to occur at least in part via the clathrin-mediated endocytic system. The protein dynamin is involved in the final stages of clathrin-coated vesicle formation. Here we show that the dynamin II isoform is expressed in 3T3-L1 adipocytes and is present in isolated plasma membrane and low density microsomal fractions. Insulin reduced the levels of dynamin II associated with the plasma membrane by about half, raising the possibility that the hormone may reduce GLUT4 endocytosis by removing dynamin from the cell surface. A fusion protein containing the amphiphysin SH3 domain selectively bound dynamin II from 3T3-L1 adipocyte cell lysates. Microinjection of the fusion protein into these cells inhibited transferrin endocytosis and increased the levels of GLUT4 at the cell surface. Glutathione S-transferase alone, the SH3 domains of spectrin and Crk, and a mutated amphiphysin SH3 domain unable to bind dynamin II did not affect GLUT4 distribution. However, a peptide containing the dynamin ...Continue Reading

References

Sep 1, 1995·The Journal of Cell Biology·B J MarshD E James
Apr 1, 1995·Endocrine Reviews·B Cheatham, C R Kahn
Nov 1, 1994·The Journal of Cell Biology·H DamkeS L Schmid
Aug 1, 1993·The Journal of Cell Biology·A M van der BliekS L Schmid
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·T A CookM A McNiven
Aug 1, 1993·The Journal of Cell Biology·J S HerskovitsR B Vallee
Sep 1, 1993·The Journal of Cell Biology·J L CarpentierJ ] Baecker J [corrected to Backer
Jan 1, 1995·Annual Review of Nutrition·M P Czech
Oct 1, 1995·Endocrine Reviews·J P Liu, P J Robinson
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·C DavidP de Camilli
May 1, 1996·The Journal of Cell Biology·J R Henley, M A McNiven
Jun 14, 1996·Biochemical and Biophysical Research Communications·O MaierP Westermann
Jun 1, 1996·The Journal of Cell Biology·K TakeiP De Camilli
Sep 1, 1996·Journal of Neurochemistry·T CookR Urrutia
Sep 1, 1996·Nature Genetics·D SakamuroG C Prendergast
Nov 15, 1996·The Journal of Biological Chemistry·H OhnoJ S Bonifacino
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·R UrrutiaM A McNiven
Apr 25, 1997·The Journal of Biological Chemistry·P M OkamotoR B Vallee
Jun 13, 1997·The Journal of Biological Chemistry·C LeprinceJ H Camonis
Jun 27, 1997·The Journal of Biological Chemistry·A R RamjaunP S McPherson
Jul 9, 1997·Biochemical and Biophysical Research Communications·K TsutsuiA Tokunaga
Aug 1, 1997·Current Biology : CB·P WiggeH T McMahon
Apr 1, 1994·Trends in Cell Biology·D E JamesJ W Slot
Mar 1, 1997·Trends in Cell Biology·M S MarksJ S Bonracino

❮ Previous
Next ❯

Citations

Jan 1, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Stephen J Royle, Ruth D Murrell-Lagnado
Oct 25, 2002·Diabetes/metabolism Research and Reviews·Silvia Mora, Jeffrey E Pessin
Dec 24, 2005·Cell and Tissue Research·Cheryl SavdieThomas Stroh
Aug 28, 1998·Current Opinion in Cell Biology·S L SchmidP De Camilli
May 14, 1999·Current Opinion in Structural Biology·J E Hinshaw
Apr 14, 1999·Trends in Cell Biology·A M van der Bliek
Jul 11, 2000·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·L H YoungR R Russell
Aug 22, 2001·The Biochemical Journal·I LisinskiH Al-Hasani
Dec 22, 1999·The Journal of Experimental Medicine·E S GoldA Aderem
Mar 10, 2006·Microbiology and Molecular Biology Reviews : MMBR·Gang RenAlan L Munn
Oct 14, 2000·Annual Review of Cell and Developmental Biology·J E Hinshaw
Nov 1, 2001·Proceedings of the National Academy of Sciences of the United States of America·J HuangJ M Olefsky
Dec 2, 2005·EMBO Reports·Chandrasagar B Dugani, Amira Klip
Jun 13, 2015·Nutrition & Metabolism·Yaqin ZhangDalong Zhu
Oct 26, 2000·Protein Expression and Purification·J DongP Westermann
Dec 17, 2003·The Journal of Biological Chemistry·Adilson GuilhermeMichael P Czech
Dec 7, 2017·Frontiers in Endocrinology·Ragadeepthi Tunduguru, Debbie C Thurmond
Aug 31, 2017·Integrative Biology : Quantitative Biosciences From Nano to Macro·Raphaël TrouillonMartin A M Gijs
Jul 13, 2004·The Journal of Biological Chemistry·Adilson GuilhermeMichael P Czech
Jun 2, 2005·American Journal of Physiology. Endocrinology and Metabolism·Gang LiuJeffrey E Pessin
Apr 15, 2004·Endocrine Reviews·Robert T WatsonJeffrey E Pessin
Dec 23, 1999·The Journal of Biological Chemistry·C W ChowJ Orlowski
May 21, 1999·The Journal of Biological Chemistry·N K HussainP S McPherson
Jan 23, 1999·The Journal of Biological Chemistry·J E PessinS Okada
Jan 25, 2000·The Journal of Biological Chemistry·L A Garza, M J Birnbaum
Dec 24, 2002·The Journal of Biological Chemistry·Satoshi ShigematsuJeffrey E Pessin
May 9, 2002·American Journal of Physiology. Renal Physiology·Tian-Xiao SunDennis Brown
Apr 26, 2000·American Journal of Physiology. Endocrinology and Metabolism·A W KaoJ E Pessin
Sep 1, 2005·Functional Plant Biology : FPB·Stephen J DibleyJohn W Patrick

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.