Perturbation of lamellar granule secretion by sodium caprate implicates epidermal tight junctions in lamellar granule function

Journal of Dermatological Science
Shohei KurodaAkemi Ishida-Yamamoto

Abstract

Polarized secretion of lamellar granules (LGs) delivers various lipids, proteases, and protease inhibitors into the stratum corneum (SC) of the epithelium. Disruption of LGs is associated with severe cutaneous diseases, but the mechanism of their polarized secretion is not known. On the other hand, recent study shows epidermal tight junctions (TJs) localize in stratum granulosum (SG), and TJs are involved in polarized molecule secretion. Thus, we hypothesized epidermal TJs relate to polarized LGs secretion. To assess the possibility that epidermal TJs are involved in polarized LGs secretion. In order to examine LGs secretion, we used fluorescent ceramide (BODIPY FL C(5)-ceramide) and a natural LG cargo, lympho-epithelial Kazal-type-related inhibitor (LEKTI), in cultured normal human epidermal keratinocytes and a reconstructed human epidermis. We investigated their alteration using the medium-chain fatty acid sodium caprate (C10), TJs inhibitor. In addition, LG distribution was observed by electron microscopy. C10 significantly inhibited secretion of both fluorescent ceramide and LEKTI in cultured normal human epidermal keratinocytes and a reconstructed human epidermis. C10 also disturbed the polarized localization of fluorescen...Continue Reading

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Citations

Mar 1, 2012·Pflügers Archiv : European journal of physiology·Naoko KidaMakoto Tominaga
May 29, 2013·Journal of Dermatological Science·Haruka GotoYasuo Kitajima
Feb 2, 2012·The Journal of Clinical Investigation·Akiharu KuboMasayuki Amagai
Jun 2, 2015·Biological & Pharmaceutical Bulletin·Yoshihiro UchinoYasufumi Kataoka
Jun 8, 2012·Annals of the New York Academy of Sciences·Nina Kirschner, Johanna M Brandner
Feb 11, 2015·Cell and Tissue Research·Johanna M Brandner, Jörg D Schulzke
Jul 15, 2017·Clinical and Experimental Immunology·P ChieosilapathamF Niyonsaba
Mar 3, 2012·Experimental Dermatology·Nina KirschnerJohanna M Brandner

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