Abstract
Agonist-induced Ca2+ oscillations in rat hepatocytes involve the production of myo-inositol-1,4,5-trisphosphate (IP3), which stimulates the release of Ca2+ from intracellular stores. The oscillatory frequency is conditioned by the agonist concentration. This study investigated the role of IP3 concentration in the modulation of oscillatory frequency by using microinjected photolabile IP3 analogs. Photorelease of IP3 during hormone-induced oscillations evoked a Ca2+ spike, after which oscillations resumed with a delay corresponding to the period set by the agonists. IP3 photorelease had no influence on the frequency of oscillations. After photorelease of 1-(alpha-glycerophosphoryl)-D-myo-inositol-4,5-diphosphate (GPIP2), a slowly metabolized IP3 analog, the frequency of oscillations initially increased by 34% and declined to its original level within approximately 6 min. Both IP3 and GPIP2 effects can be explained by their rate of degradation: the half-life of IP3, which is a few seconds, can account for the lack of influence of IP3 photorelease on the frequency, whereas the slower metabolism of GPIP2 allowed a transient acceleration of the oscillations. The phase shift introduced by IP3 is likely the result of the brief elevatio...Continue Reading
References
Jan 1, 1978·Progress in Biophysics and Molecular Biology·J W Stucki
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G W De Young, J Keizer
Feb 1, 1992·Cell Calcium·M H Nathanson, A D Burgstahler
Feb 1, 1991·Cell Calcium·A P ThomasT A Rooney
Jun 27, 1991·Nature·I BezprozvannyB E Ehrlich
Nov 18, 1991·FEBS Letters·C C PetersenO H Petersen
Apr 24, 1990·FEBS Letters·M Wakui, O H Petersen
Jul 15, 1990·The Biochemical Journal·C J DixonP H Cobbold
May 25, 1989·Nature·M WakuiO H Petersen
Nov 1, 1989·Cell Calcium·A R HughesJ W Putney
Feb 1, 1988·Pflügers Archiv : European journal of physiology·G Rapp, K Güth
May 21, 1987·Nature·J W WalkerD R Trentham
Feb 13, 1986·Nature·N M WoodsP H Cobbold
Nov 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J SneydM J Sanderson
Jul 10, 1995·FEBS Letters·J Y ChattonJ W Stucki
Jun 1, 1995·Biophysical Journal·J F WoottonD C Ogden
Jan 1, 1995·The Journal of General Physiology·S S WangS H Thompson
Feb 9, 1995·Nature·G S BirdJ W Putney
Dec 1, 1994·The Biochemical Journal·J J UblJ W Stucki
Oct 1, 1993·Endocrine Reviews·J W Putney, G S Bird
Jan 4, 1994·Biochimica Et Biophysica Acta·J W Stucki, R Somogyi
Jul 19, 1996·The Journal of Biological Chemistry·E Oancea, T Meyer
Mar 1, 1997·Cell Calcium·G S BirdJ W Putney
Jan 24, 1998·The Biochemical Journal·J Y ChattonJ W Stucki
Jul 4, 1998·Biophysical Chemistry·J SneydM J Sanderson
Citations
Feb 12, 2004·Biophysical Chemistry·Ann Zahle LarsenUrsula Kummer
Mar 23, 2011·Cold Spring Harbor Perspectives in Biology·Geneviève DupontJames W Putney
Oct 5, 2010·HFSP Journal·Geneviève Dupont, Huguette Croisier
Apr 10, 2014·Biochimica Et Biophysica Acta·Doris HöglingerCarsten Schultz
Sep 8, 2010·Mathematical Biosciences·Weijiu LiuJingvoon Chen
Mar 4, 2000·Nature Neuroscience·L G MorrisS L Hooper
Jun 4, 2004·American Journal of Physiology. Cell Physiology·Nelson P BarreraManuel Villalón
Jan 16, 2003·FEBS Letters·G DupontL Combettes
Aug 30, 2021·Progress in Neurobiology·Shaily MalikEliseo A Eugenin