Perturbation of nucleosome structure by the erythroid transcription factor GATA-1

Journal of Molecular Biology
J BoyesG Felsenfeld

Abstract

The ability of transcription factors to gain access to their sites in chromatin requires the disruption or displacement of nucleosomes covering the promoter, signalled by the generation of a nuclease hypersensitive site. We characterise here the alterations in nucleosome structure caused by binding of the erythroid factor GATA-1 to a nucleosome carrying GATA-1 sites. DNase I and micrococcal nuclease probes show that GATA-1 binding causes extensive, cooperative breakage of the histone/DNA contacts to generate a complex very similar to that formed by the factor with free DNA. The only region which differs is confined to about 50 bp surrounding the nucleosome dyad axis which appears to be the domain of residual contact between the DNA and histone octamer. Despite considerable breakage of the histone/DNA contacts, the complex is completely stable in solution, and disruption of the nucleosome is entirely reversible: it is regenerated quantitatively upon removal of the transcription factor. Moreover, the histone 2A/2B component of the octamer does not exchange to external competitor. We suggest that formation of this complex may be a step in the generation of a fully hypersensitive site in vivo over regulatory elements containing GAT...Continue Reading

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Citations

Dec 5, 2003·Nucleic Acids Research·Anan Ragab, Andrew Travers
May 18, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Kohta TakahashiShigeaki Saitoh
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·A BellG Felsenfeld
Apr 13, 2004·Eukaryotic Cell·M Isabel Muro-PastorClaudio Scazzocchio
Nov 7, 2007·Molecular and Cellular Biology·Martín Escamilla-Del-Arenal, Félix Recillas-Targa
Dec 31, 2011·Yi chuan = Hereditas·He-Yuan QiXiang-Dong Fang
Jan 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Saumen PalEmery H Bresnick
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Aug 28, 2003·The Journal of Biological Chemistry·Rodolfo Ghirlando, Cecelia D Trainor
Oct 10, 2007·The Journal of Biological Chemistry·Mitsutoki Hatta, Lisa Ann Cirillo
Sep 27, 2002·Experimental Biology and Medicine·Susanna HarjuKenneth R Peterson
Nov 27, 2019·IUBMB Life·Laura GutiérrezJohn Strouboulis
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