PMID: 8612330Nov 21, 1995Paper

Perturbation of target-directed neurite outgrowth in embryonic CNS co-cultures grown in the presence of ethanol

Brain Research. Developmental Brain Research
M B HeatonD W Walker

Abstract

Studies were conducted to determine the influence of ethanol on target-directed fiber outgrowth in culture, using embryonic chick spinal cord-muscle, and fetal rat septal-hippocampal co-cultured explants. Process extension from the spinal cord and septal explants in control cultures was selectively oriented toward the appropriate target tissue. Ethanol in the culture medium (500 mg/dl) eliminated this target-oriented outgrowth in both systems, although the overall extent of neurite outgrowth was not affected. In an effort to further characterize the source of this disruption, target explants were grown alone, with and without ethanol, and the target-conditioned culture media was subsequently harvested and placed on newly plated spinal cord or septal explants, to determine whether ethanol decreased the target production of soluble substances. To determine whether deposition of substrate-bound materials by the target tissue was affected by ethanol, spinal cord or septal explants were plated in wells which had previously been occupied by the appropriate target tissue. These studies revealed that ethanol significantly inhibited production of soluble and substrate-bound materials by muscle explants, but not by hippocampal explants. ...Continue Reading

References

Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·F Collins
Nov 29, 1991·Brain Research·R O MessingJ J Park
Feb 22, 1991·Brain Research. Developmental Brain Research·Y Arimatsu, M Miyamoto
Jun 7, 1991·Brain Research·M W Wooten, S J Ewald
Dec 1, 1990·Alcoholism, Clinical and Experimental Research·C L RandallR F Anton
Jun 1, 1988·Alcoholism, Clinical and Experimental Research·M W Miller
Jul 1, 1987·Biochemical Pharmacology·B E Leonard
Mar 1, 1986·Alcoholism, Clinical and Experimental Research·J R WestM D Cassell
Nov 3, 1973·Lancet·K L Jones, D W Smith
Mar 2, 1981·Life Sciences·T UrsoD H Van Thiel
Nov 1, 1994·Trends in Neurosciences·O LindvallM Kokaia
Oct 15, 1994·Journal of Neuroscience Research·M B HeatonD W Walker
Dec 15, 1993·Journal of Neuroscience Research·R A HughesH Thoenen
Mar 15, 1994·The Journal of Experimental Zoology·T Somasekhar, E D Pollack
Mar 19, 1993·Brain Research. Developmental Brain Research·J ZouR J Pentney

❮ Previous
Next ❯

Citations

Jan 14, 2003·Brain Research. Developmental Brain Research·David J Prince, Robert L Carlone
Aug 16, 2001·Alcoholism, Clinical and Experimental Research·H HallakR Rubin
Sep 11, 2003·Alcoholism, Clinical and Experimental Research·Hsiao-Nan HaoWilliam D Lyman
May 28, 2004·Alcohol·Stephanie M BinghamJeremy R Montague
May 1, 1999·The Journal of Biological Chemistry·C F BearerG Cheng

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.