Perturbation of the hydrophobic core of lipid bilayers by the human antimicrobial peptide LL-37

Biochemistry
Katherine A Henzler-WildmanAyyalusamy Ramamoorthy

Abstract

LL-37 is a cationic, amphipathic alpha-helical antimicrobial peptide found in humans that kills cells by disrupting the cell membrane. To disrupt membranes, antimicrobial peptides such as LL-37 must alter the hydrophobic core of the bilayer. Differential scanning calorimetry and deuterium ((2)H) NMR experiments on acyl chain perdeuterated lipids demonstrate that LL-37 inserts into the hydrophobic region of the bilayer and alters the chain packing and cooperativity. The results show that hydrophobic interactions between LL-37 and the hydrophobic acyl chains are as important for the ability of this peptide to disrupt lipid bilayers as its electrostatic interactions with the polar headgroups. The (2)H NMR data are consistent with the previously determined surface orientation of LL-37 (Henzler Wildman, K. A., et al. (2003) Biochemistry 42, 6545) with an estimated 5-6 A depth of penetration of the hydrophobic face of the amphipathic helix into the hydrophobic interior of the bilayer. LL-37 also alters the material properties of lipid bilayers, including the area per lipid, hydrophobic thickness, and coefficient of thermal expansion in a manner that varies with lipid type and temperature. Comparison of the effect of LL-37 on 1-palmit...Continue Reading

References

Mar 21, 1983·Biochimica Et Biophysica Acta·J H Davis
Feb 24, 1998·Solid State Nuclear Magnetic Resonance·M A McCabe, S R Wassall
Feb 13, 2001·Biophysical Journal·J A WhilesR R Vold
Oct 31, 2001·European Journal of Biochemistry·A GiangasperoA Tossi
Jan 7, 2003·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Gary V MartinezMichael F Brown
Feb 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna Di NardoRichard L Gallo
Mar 5, 2003·Pharmacological Reviews·Michael R Yeaman, Nannette Y Yount

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Citations

Jul 31, 2009·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Oliver Soehnlein
Oct 13, 2006·European Biophysics Journal : EBJ·Holger A ScheidtDaniel Huster
Jun 27, 2009·The Journal of Microbiology·Soon-ja KimJoong-Kook Choi
Apr 30, 2013·Colloids and Surfaces. B, Biointerfaces·Claudia DannehlGerald Brezesinski
Nov 8, 2008·Biochemistry·Ravi Prakash Reddy NangaAyyalusamy Ramamoorthy
Jan 25, 2007·Journal of the American Chemical Society·Sergey V DvinskikhAyyalusamy Ramamoorthy
May 21, 2010·Journal of the American Chemical Society·Pieter E S SmithAyyalusamy Ramamoorthy
Mar 23, 2012·Journal of the American Chemical Society·Matthew T EddyRobert G Griffin
Mar 13, 2009·Journal of the American Chemical Society·Pieter E S SmithAyyalusamy Ramamoorthy
May 22, 2009·Journal of the American Chemical Society·Ravi Prakash Reddy NangaAyyalusamy Ramamoorthy
Sep 7, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Almut MeckeMark M Banaszak Holl
May 21, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Pei YangZhan Chen
Nov 11, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Dong-Kuk LeeAyyalusamy Ramamoorthy
Mar 16, 2013·Physical Chemistry Chemical Physics : PCCP·Jeffrey R BrenderAyyalusamy Ramamoorthy
May 3, 2013·Molecular BioSystems·Jagannath JanaSubhrangsu Chatterjee
Mar 24, 2010·The Journal of Biological Chemistry·Feten AbbassiAli Ladram
Aug 5, 2004·The Journal of Biological Chemistry·Fernando PorcelliGianluigi Veglia
Sep 4, 2008·The Journal of Biological Chemistry·Linda TomasinsigMargherita Zanetti
Jun 28, 2006·Antimicrobial Agents and Chemotherapy·Joanne E ThwaiteTimothy P Atkins
May 21, 2008·Infection and Immunity·Linda JohanssonAnna Norrby-Teglund
Jul 17, 2009·BMC Research Notes·Mukesh PasupuletiArtur Schmidtchen
May 6, 2010·Journal of Leukocyte Biology·Katherine A Radek
Apr 11, 2009·PLoS Computational Biology·Ping JiangYuguang Mu
Oct 15, 2013·International Journal of Medical Microbiology : IJMM·Miriam Wilmes, Hans-Georg Sahl
Jul 12, 2007·Seminars in Immunopathology·Katherine Radek, Richard Gallo
Oct 8, 2013·Applied and Environmental Microbiology·Bianca AudrainJean-Marc Ghigo
Nov 26, 2009·Natural Product Reports·Matthew F Burton, Patrick G Steel
May 28, 2011·Natural Product Reports·Miriam WilmesKarin Thevissen
Feb 6, 2013·Cellular and Molecular Life Sciences : CMLS·Nicole L van der WeerdenMarilyn A Anderson
Mar 8, 2013·Progress in Nuclear Magnetic Resonance Spectroscopy·Ulrich H N DürrAyyalusamy Ramamoorthy
Apr 24, 2012·Developmental and Comparative Immunology·Irene de BruijnChristopher J Secombes

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