Aug 26, 1976

Perturbational effects of inorganic cations on human erythrocyte membranes

The Journal of Membrane Biology
D V Godin, M Garnett

Abstract

The perturbational effects of monovalent and divalent cations on human erythrocyte membranes were analyzed by examining their influence on kinetic and structural characteristics of trinitrobenzenesulfonic acid (TNBS) incorporation into the amino groups of protein and phospholipid structural components. The stimulatory effects of monovalent cations on TNBS incorporation, which were size-independent and attributed to nonspecific membrane alterations resulting from ionic strength factors, contrasted with the more pronounced stimulatory properties of divalent cations which were markedly size-dependent. These stimulatory effects of cations on TNBS incorporation were associated with alterations not only in rate but also in activation energy in incorporation. Changes in activation energy produced by divalent cations paralleled their ability to perturb membrane protein components and probably reflected changes in probe permeation. The rate of TNBS incorporation exhibited a dependence on divalent cation ionic radius which paralleled ion-induced perturbations in the labelling of the membrane amino phospholipid phosphatidylethanolamine. Divalent cations differed both in the relative extent and in the characteristics of protein and phospho...Continue Reading

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Citations

Mentioned in this Paper

Cations, Divalent
Nitrobenzenes
5,5'-Dithiobis(nitrobenzoate)
Cations, Monovalent
Erythrocytes
Hemolysis
Hydrogen-Ion Concentration
Plasma Membrane
Trinitrobenzenesulfonic Acid, Sodium Salt
Butylamines

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