Perturbed bone marrow monocyte development following burn injury and sepsis promote hyporesponsive monocytes

Journal of Burn Care & Research : Official Publication of the American Burn Association
Kuzhali MuthuRavi Shankar

Abstract

The mechanism of monocyte deactivation in critically injured burn patients remains unresolved. Two functionally distinct F4/80+Gr-1+ and F4/80+Gr-1- monocyte subsets have been characterized based on their homing to inflammatory or noninflammatory tissues, respectively. We hypothesized that the posttraumatic milieu in the bone marrow (BM) blunts the production of "inflammatory" monocytes. C57Blk/J male mice were divided into sham (S), burn (B), and burn sepsis (BS) groups. B and BS received a 15% dorsal scald burn and BS was inoculated with 15K colony forming units Pseudomonas aeruginosa at the burn site. Animals were killed and blood and femoral BM were collected 48, 72, and 96 hours after injury. ER-MP20 monocyte progenitors were isolated from BM and differentiated into macrophage (MØ) or dendritic cells (DCs) and characterized by the cell surface expression of F4/80 and CD11c, respectively. In both cell types, TLR-4 agonist induced cytokine levels were determined. Results showed a 2-fold increase in the F4/80+Gr-1+ subset at 48 hours in BS that started to decline at 72 hours and remained low at 96 hours. ER-MP20 progenitors isolated at 48 hours exhibited robust MØ differentiation potential but a significant decline in the per...Continue Reading

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Citations

Dec 9, 2008·Journal of Burn Care & Research : Official Publication of the American Burn Association·Kuzhali MuthuRavi Shankar
Feb 5, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Christina L Thomas-VirnigB Lynn Allen-Hoffmann
Jul 12, 2013·PloS One·Fangming XiuMarc G Jeschke
Dec 17, 2014·Journal of Burn Care & Research : Official Publication of the American Burn Association·Soman SenTina Palmieri
Aug 8, 2012·Journal of Burn Care & Research : Official Publication of the American Burn Association·Keneeshia N WilliamsKuzhali Muthumalaiappan
Jan 21, 2010·Cellular and Molecular Life Sciences : CMLS·Julie PatenaudeJacques Bernier
Aug 4, 2015·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·B ReljaI Marzi
Dec 28, 2012·Critical Care Medicine·Stephen W Bickler, Antonio De Maio
Nov 16, 2013·Frontiers of Medicine·Guangqing Wang, Zhaofan Xia
Sep 30, 2015·Journal of Laboratory Physicians·Senjuti DasguptaSudipta Chakrabarti
Apr 21, 2012·The Journal of Biological Chemistry·David M CauviAntonio De Maio
Mar 20, 2016·Journal of Leukocyte Biology·Nicholas B JohnsonKuzhali Muthumalaiappan
Feb 15, 2020·Nature Reviews. Disease Primers·Marc G JeschkeSarvesh Logsetty
Apr 5, 2019·Proceedings of the National Academy of Sciences of the United States of America·Todd W CostantiniAndrew Baird
Jul 18, 2021·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Helen WilliamsHeather J Medbury
Jan 23, 2021·Shock·Marisa E LuckMashkoor A Choudhry

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