Pertussis toxin inhibits EGF-, phorbol ester- and insulin-stimulated DNA synthesis in BALB/c3T3 cells: evidence for post-receptor activation of Gi alpha

Biochemical and Biophysical Research Communications
M F CrouchI A Hendry

Abstract

The contribution of the GTP-binding protein, Gi, to EGF, phorbol dibutyrate (PdBu)-, and insulin-stimulated DNA synthesis was examined in BALB/c3T3 cells. Pertussis toxin inhibited DNA synthesis by each agonist, particularly at suboptimal agonist concentrations, but the inhibition could be partially overcome with higher agonist concentrations and combinations of these agonists. This suggested that (1) some, but not all, of the mitogenic signals for all three agonists were transduced by Gi (2) Gi may be activated by post-receptor mechanisms involving protein kinase C. Gi alpha-specific antibodies and ADP-ribosylation by pertussis toxin using 32P-NAD each labelled a single protein band, representing one or more species of Gi alpha. Pertussis toxin treatment increased the synthesis of Gi alpha. These results are discussed in relation to possible direct effects of Gi alpha on nuclear control during division.

References

Jan 1, 1987·Molecular and Cellular Endocrinology·A M Spiegel
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M F CrouchE G Lapetina
Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
May 3, 1988·Biochemistry·Y Yarden, A Ullrich
Jan 1, 1985·Journal of Neuroscience Research·R E BonyhadyD J Watters
Sep 17, 1984·Biochemical and Biophysical Research Communications·L M Vicentini, M L Villereal

❮ Previous
Next ❯

Citations

May 1, 1995·Cellular Signalling·I RamírezM Soley
Feb 8, 2003·Brain Research. Molecular Brain Research·Pilar Sánchez-BlázquezJavier Garzón
Aug 18, 2000·Immunology and Cell Biology·F S Willard, M F Crouch
Dec 1, 1995·Australian and New Zealand Journal of Medicine·M F CrouchI A Hendry
Mar 1, 1994·American Journal of Reproductive Immunology : AJRI·S FuruyaS Nozawa
Sep 27, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Claudie HecquetErvin G Erdös
Jul 1, 1996·Neurochemical Research·S O JohansonI A Hendry
Apr 30, 2011·Physiological Reviews·Helmut KettenmannAlexei Verkhratsky
Apr 7, 1997·Biochemical and Biophysical Research Communications·M F Crouch

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.