Pertussis toxin-insensitive effects of mastoparan, a wasp venom peptide, in PC12 cells

Journal of Cellular Physiology
T MurayamaY Nomura

Abstract

Recent studies have shown that mastoparan, an amphiphilic peptide derived from wasp venom, modifies the secretion of neurotransmitters and hormones from a variety of cell types. Mastoparan interacts with heterotrimeric guanine nucleotide-binding proteins (G proteins) such as Gi and G(o), which are ADP-ribosylated by pertussis toxin (PTX) and thereby uncoupled from receptors. Previously, some of the effects of mastoparan including secretion were reported to be modified selectively by PTX but not by cholera toxin (CTX). In the present study, we examined the influence of bacterial toxins on the effects of mastoparan in PC12 cells. Mastoparan stimulated [3H]noradrenaline (NA) release from prelabeled PC12 cells in the absence of CaCl2, although high K+ or ATP-stimulated the release in a Ca(2+)-dependent manner. Pretreatment with CTX, not PTX, for 24 h inhibited mastoparan-stimulated [3H]NA release. Mastoparan inhibited forskolin-stimulated cyclic AMP accumulation in a dose-dependent manner, although mastoparan had no effect by itself. Pretreatment with PTX completely abolished the inhibitory effect of carbachol via Gi on cyclic AMP accumulation and partially reduced the effect of mastoparan. However, the inhibitory effect of 20 micr...Continue Reading

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Citations

Jul 3, 1998·European Journal of Pharmacology·T MurayamaY Nomura
Jun 24, 1998·Toxicon : Official Journal of the International Society on Toxinology·K MizunoY Ohizumi
Apr 7, 1999·Archives of Biochemistry and Biophysics·T NaganumaY Nomura
Jun 20, 1998·Archives of Biochemistry and Biophysics·T MurayamaY Nomura

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