PMID: 2505888Aug 21, 1989Paper

Pertussis toxin lesioning of the nucleus caudate-putamen attenuates adenylate cyclase inhibition and alters neuronal electrophysiological activity

Brain Research
C BoyajianD M Cooper

Abstract

Receptor-mediated inhibition of brain adenylate cyclase activity has been well characterized at the biochemical level. However, less understood is how these, typically modest, inhibitory effects on cyclase activity correlate with the electrophysiological activity of brain preparations. In addressing this question, we injected pertussis toxin (PT) into the nucleus caudate-putamen of intact rats, and observed a subsequent attenuated inhibition of adenylate cyclase activity in caudate membranes, which correlated with altered electrophysiological activity in this nucleus. PT completely abolished and electrophysiological activity in this nucleus. PT completely abolished and significantly reduced, respectively, dopamine D2 and opioid receptor-mediated inhibition of adenylate cyclase. In addition, pretreatment of rat caudate nuclei with PT attenuated the amount of in vitro ADP-ribosylation of 41,000 and 39,000 Da PT substrates measured in caudate membranes. Extracellular recording of the spontaneous activity of caudate neurons revealed that PT pretreatment significantly increased firing rates above those of cells recorded from sham-operated and unoperated controls. Furthermore, a significantly greater number of cells pretreated with P...Continue Reading

References

Jan 1, 1987·Molecular and Cellular Endocrinology·A M Spiegel
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R A NorthM J Christie
Aug 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L E Limbird
Oct 1, 1986·Physiology & Behavior·J P Kroon, A L Riley
Apr 1, 1974·Analytical Biochemistry·Y SalomonM Rodbell
Feb 22, 1982·FEBS Letters·D M Cooper
Jul 1, 1983·Neuropharmacology·S W JohnsonR Freedman

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Citations

Mar 19, 1991·European Journal of Pharmacology·C MarinT N Chase
Dec 3, 2010·Cold Spring Harbor Perspectives in Biology·Michelle L Halls, Dermot M F Cooper

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