Pertussis toxin-mediated ADP-ribosylation of rabbit luteal Gi uncouples enkephalin inhibition of adenylyl cyclase

The International Journal of Biochemistry
J AbramowitzB P Jena

Abstract

1. Some of the actions of pertussis toxin on the rabbit luteal adenylyl cyclase system were analyzed. 2. Incubation of luteal membranes with pertussis toxin and [32P]NAD resulted in the [32P]ADP-ribosylation of a 40,000 Da protein that is distinct from the proteins ADP-ribosylated by cholera toxin. 3. Pertussis toxin specific [32P]ADP-ribosylation was time-dependent and dependent upon the concentration of pertussis toxin present during the incubation. 4. Pertussis toxin mediated [32P]ADP-ribosylation was enhanced by ATP, ADP, adenylyl imidodiphosphate, GTP, guanosine-5'-O-(2-thiodiphosphate), guanosine-5'-O-(3-thiotriphosphate), and NaF but not AMP or guanylyl imidodiphosphate [GMP-P(NH)P]. 5. Treatment of luteal membranes with NAD and pertussis toxin prevents GTP and enkephalin but not GMP-P(NH)P mediated inhibition of forskolin stimulated adenylyl cyclase, demonstrating the existence of a functional Gi in the rabbit corpus luteum.

References

Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D Cassel, T Pfeuffer
Apr 1, 1974·Analytical Biochemistry·Y SalomonM Rodbell
Oct 1, 1982·Biology of Reproduction·T Shu-DongC W Bardin
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May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T Katada, M Ui

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Citations

Jan 6, 2000·FEBS Letters·O N Solovjeva, G A Kochetov
Dec 31, 1997·FEBS Letters·M V KovinaG A Kochetov
May 31, 2006·Biochemistry. Biokhimii︠a︡·I A SevostyanovaG A Kochetov

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