PMID: 2505676Oct 1, 1989Paper

Pertussis toxin-sensitive GTP-binding proteins may regulate phospholipase A2 in response to thrombin in rabbit platelets

Archives of Biochemistry and Biophysics
Y KajiyamaY Nomura

Abstract

Incubation of rabbit platelets with thrombin resulted in rapid accumulations of inositol trisphosphate (IP3) in [3H]inositol-labeled platelets, increases of [3H]arachidonic acid [( 3H]AA) release, and [3H]serotonin secretion from the platelets prelabeled with these labeled compounds. The experiments using phospholipase A2 or C inhibitor suggested that not only phospholipase C but also phospholipase A2 activity plays an important role in serotonin secretion. We then studied the regulatory mechanisms of phospholipase A2 activity. Guanosine 5'-(3-O-thio)triphosphate (GTP gamma S), guanyl-5'-(beta,gamma-iminio)triphosphate), or AlF4- caused a significant liberation of AA in digitonin-permeabilized platelets but not in intact platelets. Thrombin-stimulated AA release was not observed in permeabilized platelets, whereas thrombin acted synergistically with GTP or GTP analogs to stimulate AA release. GTP analog-stimulated AA release was inhibited by guanosine 5'-(2-O-thio)diphosphate) and was also inhibited by decreased Mg2+ concentrations. Thrombin-induced, GTP-dependent AA release, but not IP3 formation, was diminished by 100 ng/ml of pertussis toxin, associated with ADP-ribosylation of membrane 41-kDa protein(s). Thrombin-stimulated...Continue Reading

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Citations

Apr 1, 1994·Molecular and Cellular Endocrinology·S Offermanns, G Schultz
Aug 14, 1992·Biochimica Et Biophysica Acta·S Cockcroft
Nov 30, 1995·European Journal of Pharmacology·S AkibaT Sato
Mar 1, 1991·Trends in Cardiovascular Medicine·L F Brass, D R Manning
Oct 24, 1998·The International Journal of Biochemistry & Cell Biology·R N Puri
Mar 1, 1992·American Journal of Respiratory Cell and Molecular Biology·J G GarciaJ Dominguez
Jul 31, 1990·Biochemical and Biophysical Research Communications·Y OzakiS Kume
Apr 14, 1994·Biochimica Et Biophysica Acta·J H Exton
Jan 1, 1989·Progress in Lipid Research·H AritaK Hanasaki
Feb 28, 1990·Biochemical and Biophysical Research Communications·S A Metz, M Dunlop

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