Pertussis toxin-sensitive GTP-binding proteins characterized in synaptosomal fractions of embryonic avian cerebral cortex

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
G G Holz, T J Turner

Abstract

Pertussis toxin (PTX)-sensitive GTP-binding proteins (G proteins) are essential intermediaries subserving neuronal signal transduction pathways that regulate excitation-secretion coupling. Despite this established role, relatively little is known regarding the identity, subcellular distribution, and relative abundance of this class of G proteins in synaptic nerve endings. Here, sucrose density gradient centrifugation was combined with 1- and 2-dimensional gel electrophoresis to characterize PTX-sensitive G protein alpha subunits in synaptosomal fractions of embryonic (day 12) chick cerebral cortical homogenates. These findings demonstrate multiple isoforms of M(r) 40-41 kDa Gi alpha and G(o) alpha subunits that can be identified on the basis of PTX-catalyzed ADP-ribosylation and immunoblot analysis.

References

Feb 10, 1976·Biochemistry·G F Ames, K Nikaido
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M StrathmannM I Simon
Nov 1, 1985·Naunyn-Schmiedeberg's Archives of Pharmacology·C AllgaierG Hertting
May 16, 1988·Biochemical and Biophysical Research Communications·P BrabetV Homburger
Jul 12, 1988·Biochemistry·M A Lochrie, M I Simon
Feb 1, 1964·The Biochemical Journal·V P WhittakerR J Kirkland
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T Katada, M Ui
Jan 1, 1994·Methods in Enzymology·D J Carty

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