Oct 23, 2018

Pervasive and Dynamic Transcription Initiation in Saccharomyces cerevisiae

BioRxiv : the Preprint Server for Biology
Zhaolian Lu, Zhenguo Lin


Transcription initiation is finely regulated to ensure the proper expression and function of eukaryotic genes. The regulated transcription initiation in response to various environmental cues in the model organism Saccharomyces cerevisiae has not been systematically investigated. In this study, we generated quantitative maps of transcription start site (TSS) at a single-nucleotide resolution for S. cerevisiae grown in nine different conditions using no-amplification non-tagging Cap analysis of gene expression (nAnT-iCAGE) sequencing. Based on 337 million uniquely mapped CAGE tags, we mapped ~1 million well-supported TSSs, suggesting highly pervasive transcription initiation in the compact genome of yeast. The comprehensive TSS maps allowed us to identify core promoters for ~96% verified protein-coding genes and to revise the predicted translation start codon for 183 genes. We found that 56% of yeast genes have at least two core promoters and alternative usage of different core promoters in a gene is widespread in response to changing environments. More importantly, most core promoter shifts are coupled with differential gene expression, indicating that core promoter shift might play an important role in controlling transcriptio...Continue Reading

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Mentioned in this Paper

Toxic Shock Syndrome
Transcriptional Regulation
Saccharomyces cerevisiae allergenic extract
Transcription Initiation Site
Molecular Probe Techniques
Transcription, Genetic

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