Pervasive, Coordinated Protein-Level Changes Driven by Transcript Isoform Switching during Meiosis

Cell
Ze ChengGloria A Brar

Abstract

To better understand the gene regulatory mechanisms that program developmental processes, we carried out simultaneous genome-wide measurements of mRNA, translation, and protein through meiotic differentiation in budding yeast. Surprisingly, we observed that the levels of several hundred mRNAs are anti-correlated with their corresponding protein products. We show that rather than arising from canonical forms of gene regulatory control, the regulation of at least 380 such cases, or over 8% of all measured genes, involves temporally regulated switching between production of a canonical, translatable transcript and a 5' extended isoform that is not efficiently translated into protein. By this pervasive mechanism for the modulation of protein levels through a natural developmental program, a single transcription factor can coordinately activate and repress protein synthesis for distinct sets of genes. The distinction is not based on whether or not an mRNA is induced but rather on the type of transcript produced.

Citations

Feb 9, 2019·RNA·Max B Ferretti, Katrin Karbstein
Jul 25, 2018·Cold Spring Harbor Perspectives in Biology·Nicholas T IngoliaJonathan S Weissman
May 28, 2019·Molecular & Cellular Proteomics : MCP·Burcu VitrinelChristine Vogel
Jan 11, 2020·Genes & Development·Jingxun ChenElçin Ünal
Feb 27, 2020·Molecular Biology and Evolution·Luke J Kosinski, Joanna Masel
Mar 4, 2020·Journal of Bacteriology·Heather A FeagaJonathan Dworkin
Dec 13, 2018·The Journal of Cell Biology·Eric M SawyerElçin Ünal
Mar 28, 2019·Nucleic Acids Research·Daniel A SantosJonathan S Weissman
Oct 10, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ashley M NelsonChristine Milcarek
Apr 21, 2018·Current Genetics·George Maxwell Otto, Gloria Ann Brar
Aug 7, 2020·Wiley Interdisciplinary Reviews. RNA·Huy-Dung HoangTommy Alain
Aug 4, 2018·Essays in Biochemistry·Ezra Levy, Nikolai Slavov
Oct 26, 2018·Genome Research·Michael A SchonMichael D Nodine
Feb 6, 2020·Genes & Development·Régis E Meyer, Dean S Dawson
Mar 28, 2020·G3 : Genes - Genomes - Genetics·Victoria JorgensenElçin Ünal
Dec 3, 2020·American Journal of Respiratory Cell and Molecular Biology·Sang-Nam LeeJoo-Heon Yoon
Aug 9, 2019·Molecular & Cellular Proteomics : MCP·Burcu VitrinelChristine Vogel
Feb 19, 2021·Nature Communications·Hong ZhangJian Lu
Jan 28, 2021·ELife·Nikhil R BhagwatNeil Hunter
Aug 18, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Sabeeha S MerchantCrysten E Blaby-Haas
May 26, 2020·Journal of Proteome Research·Xiongwen CaoSarah A Slavoff
Oct 18, 2020·Cell·Alexander F Palazzo, Eugene V Koonin
Aug 21, 2020·Experimental Cell Research·Peter F RenzAtaman Sendoel
Feb 7, 2019·G3 : Genes - Genomes - Genetics·Ina HollererGloria A Brar
Sep 4, 2020·Experimental Cell Research·Jay S GoodmanElçin Ünal
Oct 5, 2021·Nucleic Acids Research·Artyom A EgorovSergey E Dmitriev
Dec 4, 2019·Cell·Ana FiszbeinChristopher B Burge

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.