Pest and disease protection conferred by expression of barley β-hordothionin and Nicotiana alata proteinase inhibitor genes in transgenic tobacco

Functional Plant Biology : FPB
Julia A CharityT J V Higgins

Abstract

Proteinase inhibitors and thionins are among the many proteins thought to have a role in plant defence against pests and pathogens. Complementary DNA clones encoding the precursors of a multi-domain proteinase inhibitor from Nicotiana alata Link et Otto (NA-PI) (Mr approximately 43 000) and a β-hordothionin (β-HTH) (Mr approximately 13 000) from barley, were linked to constitutive promoters and subsequently transferred by Agrobacterium-mediated transformation into tobacco. The NA-PI and β-HTH precursor proteins were synthesised and post-translationally processed in transgenic tobacco and accumulated as polypeptides of apparent size Mr approximately 6000 and Mr approximately 8500, respectively. The na-pi and β-hth genes were stably inherited for at least two generations. Transgenic tobacco plants containing the highest amounts of NA-PI and β-HTH were crossed to produce plants containing both genes. Helicoverpa armigera (tobacco budworm) larvae that ingested transgenic tobacco leaves expressing both NA-PI and β-HTH, exhibited higher mortality and slower development relative to larvae fed on non-transgenic tobacco. NA-PI and β-HTH, either alone, or in combination, also conferred protection against the fungal pathogen, Botrytis cin...Continue Reading

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Citations

Sep 19, 2006·Biochemistry. Biokhimii︠a︡·V V Mosolov, T A Valueva
Mar 27, 2015·Annals of Botany·Timothy L FitzgeraldKemal Kazan
Feb 1, 2008·Plant Signaling & Behavior·Céline ChassotJean-Pierre Métraux
Jan 30, 2007·The Plant Journal : for Cell and Molecular Biology·Céline ChassotJean-Pierre Métraux
Jun 20, 2019·Protein and Peptide Letters·Kaushik DasSwapan K Datta
Feb 12, 2017·Biotechnology Letters·Shazia RehmanTariq Mahmood
Aug 5, 2008·Plant Physiology and Biochemistry : PPB·Jan SelsMiguel F C De Bolle

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