PET and planar imaging of tumor hypoxia with labeled metronidazole

Academic Radiology
Megumi ItoE E Kim

Abstract

This study was aimed to develop 99mTc- and 68Ga-labeled metronidazole (MN) using ethylenedicysteine (EC) as a chelator and evaluate their potential use to assess tumor hypoxia. EC-MN was labeled with 99mTc in the presence of tin (II) chloride. Labeling EC-MN with 68Ga was achieved by adding 68GaCl3 (2 mCi with 3.4 microg cold GaCl3). In vitro cellular uptakes of 99mTc- and 68Ga-EC-MN were obtained in various types of tumor cells at 0.5-4 hours. Tissue distribution and PET imaging of 99mTc and 68Ga-EC-MN were evaluated in breast tumor-bearing rats at 0.5-4 hours. Tumor oxygen tension was measured using an oxygen probe. There were similar cellular uptakes (2-10%) between 99mTc- and 68Ga-EC-MN at 0.5-4 hours. In vivo biodistribution of 99mTc- and 68Ga-EC-MN in breast tumor-bearing rats showed increased tumor-to-blood and tumor-to-muscle count density ratios as a function of time. Positron emission tomography images confirmed that the tumors could be visualized clearly with 68Ga-EC-MN. Oxygen tension in tumor tissue was determined to be 6-10 mm Hg compared with 40-50 mm Hg in normal muscle tissue. The results indicated that it is feasible to use 99mTc- and 68Ga-EC-MN for assessment of tumor hypoxia. These agents may be useful in se...Continue Reading

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Citations

Aug 24, 2007·European Journal of Nuclear Medicine and Molecular Imaging·Adil Al-NahhasDomenico Rubello
May 31, 2012·Journal of Biomedicine & Biotechnology·Yin-Han ZhangDavid J Yang
May 25, 2012·The British Journal of Radiology·I Mendichovszky, A Jackson
Dec 1, 2010·Nuclear Medicine and Molecular Imaging·Dinesh ShettyJae Min Jeong
May 4, 2013·Advances in Medical Sciences·A SadowskaH Car
Feb 10, 2009·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·M U KhanA Al-Nahhas
Nov 1, 2011·International Journal of Cancer. Journal International Du Cancer·Marie Van de PuttePeter A M de Witte

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