PET imaging of a collagen matrix reveals its effective injection and targeted retention in a mouse model of myocardial infarction

Biomaterials
Ali AhmadiErik J Suuronen

Abstract

Injectable biomaterials have shown promise for cardiac regeneration therapy. However, little is known regarding their retention and distribution upon application in vivo. Matrix imaging would be useful for evaluating these important properties. Herein, hexadecyl-4-[(18)F]fluorobenzoate ((18)F-HFB) and Qdot labeling was used to evaluate collagen matrix delivery in a mouse model of myocardial infarction (MI). At 1 wk post-MI, mice received myocardial injections of (18)F-HFB- or Qdot-labeled matrix to assess its early retention and distribution (at 10 min and 2h) by positron emission tomography (PET), or fluorescence imaging, respectively. PET imaging showed that the bolus of matrix at 10 min redistributed evenly within the ischemic territory by 2h. Ex vivo biodistribution revealed myocardial matrix retention of ∼ 65%, which correlated with PET results, but may be an underestimate since (18)F-HFB matrix labeling efficiency was ∼ 82%. For covalently linked Qdots, labeling efficiency was ∼ 96%. Ex vivo Qdot quantification showed that ∼ 84% of the injected matrix was retained in the myocardium. Serial non-invasive PET imaging and validation by fluorescence imaging confirmed the effectiveness of the collagen matrix to be retained and ...Continue Reading

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Citations

Jul 6, 2015·European Journal of Nuclear Medicine and Molecular Imaging·Etienne CroteauRobert A deKemp
Nov 17, 2017·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Louise KiruGuillem Pratx
Apr 2, 2019·Critical Reviews in Biotechnology·Drew KuraitisErik J Suuronen
Aug 23, 2020·International Journal of Molecular Sciences·Saltanat SmagulArman Saparov
Oct 20, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Nicol GuidolinZsolt Baranyai
Feb 16, 2021·Advanced Healthcare Materials·Maaike J G SchotmanPatricia Y W Dankers
Feb 14, 2018·Biomacromolecules·Charmainne CrujeElizabeth R Gillies

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