PET Imaging of the Natural Killer Cell Activation Receptor NKp30.

Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
Travis M ShafferSanjiv S Gambhir

Abstract

Redirecting the immune system in cancer treatment has led to remarkable responses in a subset of patients. Natural killer (NK) cells are innate lymphoid cells being explored as they engage tumor cells in different mechanisms compared with T cells, which could be exploited for treatment of nonresponders to current immunotherapies. NK cell therapies are monitored through measuring peripheral NK cell concentrations or changes in tumor volume over time. The former does not detect NK cells at the tumor site, and the latter is inaccurate for immunotherapies because of pseudoprogression. Therefore, new imaging methods are required as companion diagnostics for optimizing immunotherapies. Methods: In this study, we developed and completed preclinical in vivo validation of 2 antibody-based PET probes specific for NKp30, an activation natural cytotoxicity receptor expressed by human NK cells. Quantitative, multicolor flow cytometry during a variety of NK cell activation conditions was completed on primary human NK cells and the NK92MI cell line. Human renal cell carcinoma (RCC) tumors were stained for the NK cell receptors CD56, NKp30, and NKp46 to determine expression on tumor-infiltrating NK cells. An NKp30 antibody was radiolabeled wit...Continue Reading

Citations

Aug 2, 2020·British Journal of Pharmacology·Pedro F PinheiroM Matilde Marques
Apr 20, 2021·Frontiers in Oncology·Huaru ZhangGuosheng Yang
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Virginia LiberiniDésirée Deandreis

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