PET with 64Cu-histidine for noninvasive diagnosis of biliary copper excretion in Long-Evans cinnamon rat model of Wilson disease.

Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
Ralf BahdeSanjeev Gupta

Abstract

Excretion of copper into bile requires the copper transporter Atp7b, which is deficient in Wilson disease. We hypothesized that a radiocopper-histidine complex would be effective for diagnosing Wilson disease by molecular imaging and tested this hypothesis in the Long-Evans cinnamon (LEC) rat model with Atp7b deficiency. We complexed (64)Cu to l-histidine and analyzed clearance from blood, uptake in tissues, and excretion in bile of healthy Long-Evans agouti (LEA) rats versus LEC rats modeling Wilson disease. Sixty-minute dynamic PET recordings were obtained in LEA and LEC rats. Possible effects of acute and chronic liver injury induced by carbon tetrachloride were studied in LEA rats. Atp7b deficiency in LEC rats was reconstituted by transplantation of healthy cells to establish the specificity of findings. Examination of blood, tissue, and bile showed that in healthy rats, radiocopper was incorporated in the liver, followed by rapid excretion in bile. Corresponding blood, tissue, and bile studies in LEC rats showed incorporation of radiocopper in the liver but without copper excretion in bile, leading to hepatic retention of the radiotracer. PET showed onset of copper clearance in the liver of LEA rats, whereas liver copper c...Continue Reading

References

Oct 1, 1987·Journal of Inorganic Biochemistry·H J McArdleD M Danks
Nov 11, 1965·The American Journal of Physiology·C A Owen
Oct 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M J Bingham, H J McArdle
Aug 31, 2000·Nature Structural Biology·A K WernimontA C Rosenzweig
Mar 29, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·A N IraniS Gupta
Oct 26, 2002·Epilepsy Research·Takafumi KuzuyaToshitaka Nabeshima
May 11, 2004·The Journal of Biological Chemistry·Reiko Sanokawa-AkakuraRaymond B Birge
May 25, 2004·Inorganic Chemistry·Patrick DeschampsBibudhendra Sarkar
May 4, 2005·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Karl-Dimiter BissigMarc Solioz
Jun 14, 2005·Brain Research. Molecular Brain Research·Samreen AhmedJimo Borjigin
Oct 27, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Daniel BentenSanjeev Gupta
Feb 6, 2007·Lancet·Aftab AlaMichael L Schilsky
Feb 19, 2008·Nature Chemical Biology·Byung-Eun KimDennis J Thiele
Mar 5, 2008·Nuclear Medicine Communications·Brigid JosephSanjeev Gupta
Feb 3, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Brigid JosephSanjeev Gupta
Jun 16, 2009·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Kuldeep K BhargavaSanjeev Gupta
Feb 18, 2011·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Fangyu PengOtto Muzik

❮ Previous
Next ❯

Citations

Mar 19, 2014·Annals of the New York Academy of Sciences·Fangyu Peng
Apr 29, 2015·Journal of Hepatology·Stuart J ForbesAnil Dhawan
Feb 9, 2018·Liver International : Official Journal of the International Association for the Study of the Liver·Anna CzłonkowskaTomasz Litwin
Feb 24, 2018·Journal of Neurochemistry·Emily ReedOliver Bandmann
Mar 19, 2014·Journal of Labelled Compounds & Radiopharmaceuticals·Rebekka Hueting
May 14, 2014·Annals of the New York Academy of Sciences·Sanjeev Gupta
Jul 7, 2018·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Joanna J Bartnicka, Philip J Blower

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.