DOI: 10.1101/463711Nov 7, 2018Paper

PETISCO is a novel protein complex required for 21U RNA biogenesis and embryonic viability

BioRxiv : the Preprint Server for Biology
Ricardo J Cordeiro RodriguesRené F Ketting

Abstract

Piwi proteins are important for germ cell development in almost all animals studied thus far. These proteins are guided to specific targets, such as transposable elements, by small guide RNAs, often referred to as piRNAs, or 21U RNAs in C. elegans. In this organism, even though genetic screens have uncovered a number of potential 21U RNA biogenesis factors, little is known about how these factors interact or what they do. Based on the previously identified 21U biogenesis factor PID-1, we here define a novel protein complex, PETISCO, that is required for 21U RNA biogenesis. PETISCO contains both potential 5-cap and 5-phosphate RNA binding domains, suggesting involvement in 5 end processing. We define the interaction architecture of PETISCO and reveal a second function for PETISCO in embryonic development. This essential function of PETISCO is not mediated by PID-1, but by TOST-1. Vice versa, TOST-1 is not involved in 21U RNA biogenesis. Both PID-1 and TOST-1 are small, intrinsically disordered proteins that interact directly with the PETISCO protein ERH-2 (enhancer of rudimentary homolog 2) using a conserved sequence motif. Finally, our data suggest an important role for TOST-1:PETISCO in SL1 homeostasis in the early embryo. Our...Continue Reading

Citations

Jan 18, 2019·Non-coding RNA·Miguel Vasconcelos AlmeidaRené F Ketting

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Methods Mentioned

BETA
RNAseq
RIP
pull-downs
pull-down
RIPseq
RIPs
Y2H
transgenic
two-hybrid

Software Mentioned

SL1
RIPseq
ESPrit
PETISCO
MUSCLE
miniMos

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