Petite mutation in aged and oxidatively stressed ale and lager brewing yeast

Letters in Applied Microbiology
B R GibsonK A Smart

Abstract

To determine the role of oxidative stress and chronological ageing on the propensity of brewing yeast strains to form respiratory deficient 'petites'. Four industrial yeast strains (two ale and two lager strains) were exposed to oxidative stress in the form of H(2)O(2) (5 mmol l(-1)) for two hours. Cell viability and occurrence of petites were determined by the slide culture and TTC-overlay techniques, respectively. Increases in petite frequency were observed but only in those strains sensitive to oxidative stress. Chronological ageing under aerobic conditions led to an increase in petites in strains sensitive to oxidative stress. No such increase was observed under anaerobic conditions. Ageing may contribute to mitochondrial DNA damage and increase the propensity of brewing yeast cells to become respiratory deficient. Tolerant strains may be less likely to generate petites as a result of serial re-pitching. Continuous re-use of brewing yeast is associated with an increase in the frequency of petites within brewery yeast slurries, a phenomenon resulting in reduced fermentative capacity. The cause of petite generation during brewery handling is unknown. We show that endogenous oxidative stress has the potential to generate petit...Continue Reading

References

Dec 1, 1988·Applied and Environmental Microbiology·J JiménezT Benítez
Jan 1, 1980·Experimental Gerontology·J MiquelJ E Johnson
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·M K ShigenagaB N Ames
Feb 1, 1993·Archives of Biochemistry and Biophysics·V CostaP Moradas-Ferreira
Mar 1, 1993·Journal of General Microbiology·J Flattery-O'BrienI W Dawes
Jan 1, 1997·Applied and Environmental Microbiology·J I Ibeas, J Jimenez
Mar 8, 2000·Experimental Gerontology·R NestelbacherM Breitenbach
Nov 4, 2000·The Biochemical Journal·K SugiyamaY Inoue
Dec 18, 2001·Mechanisms of Ageing and Development·S M Van ZandyckeK A Smart
Sep 27, 2002·Experimental Biology and Medicine·Yau-Huei Wei, Hsin-Chen Lee
Jul 11, 2003·Free Radical Biology & Medicine·Mario H BarrosAlicia J Kowaltowski
Jul 16, 2003·Biochemical and Biophysical Research Communications·Lei ZhangAkinori Ohta
Jun 1, 2005·Molecular and Cellular Biology·Nicole A DoudicanPaul W Doetsch
Jul 25, 2007·FEMS Microbiology Reviews·Brian R GibsonKatherine A Smart

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Citations

Jan 19, 2011·Applied Microbiology and Biotechnology·Eduardo T V TrevisolElis C A Eleutherio
Mar 19, 2014·BMC Research Notes·Jennifer SpencerDarren Greetham
Jul 31, 2013·Microbiological Research·Elena I Zubko, Mikhajlo K Zubko
Jul 21, 2011·Asian Pacific Journal of Tropical Medicine·D IshalekuR S Houmsou
Apr 20, 2019·Frontiers in Genetics·Arthur R Gorter de VriesJean-Marc G Daran
Jun 13, 2020·Food Research International·R M Christofoleti-FurlanL C Basso

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