Petunia phospholipase c1 is involved in pollen tube growth.

The Plant Cell
Peter E DowdSimon Gilroy

Abstract

Although pollen tube growth is essential for plant fertilization and reproductive success, the regulators of the actin-related growth machinery and the cytosolic Ca2+ gradient thought to determine how these cells elongate remain poorly defined. Phospholipases, their substrates, and their phospholipid turnover products have been proposed as such regulators; however, the relevant phospholipase(s) have not been characterized. Therefore, we cloned cDNA for a pollen-expressed phosphatidylinositol 4,5-bisphosphate (PtdInsP2)-cleaving phospholipase C (PLC) from Petunia inflata, named Pet PLC1. Expressing a catalytically inactive form of Pet PLC1 in pollen tubes caused expansion of the apical Ca2+ gradient, disruption of the organization of the actin cytoskeleton, and delocalization of growth at the tube tip. These phenotypes were suppressed by depolymerizing actin with low concentrations of latrunculin B, suggesting that a critical site of action of Pet PLC1 is in regulating actin structure at the growing tip. A green fluorescent protein (GFP) fusion to Pet PLC1 caused enrichment in regions of the apical plasma membrane not undergoing rapid expansion, whereas a GFP fusion to the PtdInsP2 binding domain of mammalian PLC delta1 caused e...Continue Reading

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Citations

May 22, 2010·Sexual Plant Reproduction·Yan Zhang, Sheila McCormick
Jan 22, 2010·Protoplasma·Till IschebeckIngo Heilmann
Nov 30, 2011·Plant Molecular Biology·Sunny D Rupwate, Ram Rajasekharan
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Dec 23, 2008·Journal of Lipid Research·Teun Munnik, Christa Testerink
Oct 24, 2013·Journal of Experimental Botany·Juan David RejónAntonio Jesús Castro
Jan 20, 2016·Progress in Lipid Research·Yueyun HongXuemin Wang
May 5, 2012·Progress in Lipid Research·Jean-Luc CacasSébastien Mongrand
Mar 13, 2012·Plant Science : an International Journal of Experimental Plant Biology·Weike PeiHaiyun Ren
Jul 22, 2011·Current Opinion in Plant Biology·Teun Munnik, Erik Nielsen

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