PfEMP1 A-Type ICAM-1-Binding Domains Are Not Associated with Cerebral Malaria in Beninese Children.

MBio
V JosteGwladys I Bertin

Abstract

PfEMP1 is the major antigen involved in Plasmodium falciparum-infected erythrocyte sequestration in cerebrovascular endothelium. While some PfEMP1 domains have been associated with clinical phenotypes of malaria, formal associations between the expression of a specific domain and the adhesion properties of clinical isolates are limited. In this context, 73 cerebral malaria (CM) and 98 uncomplicated malaria (UM) Beninese children were recruited. We attempted to correlate the cytoadherence phenotype of Plasmodium falciparum isolates with the clinical presentation and the expression of specific PfEMP1 domains. Cytoadherence level on Hbec-5i and CHO-ICAM-1 cell lines and var genes expression were measured. We also investigated the prevalence of the ICAM-1-binding amino acid motif and dual receptor-binding domains, described as a potential determinant of cerebral malaria pathophysiology. We finally evaluated IgG levels against PfEMP1 recombinant domains (CIDRα1.4, DBLβ3, and CIDRα1.4-DBLβ3). CM isolates displayed higher cytoadherence levels on both cell lines, and we found a correlation between CIDRα1.4-DBLβ1/3 domain expression and CHO-ICAM-1 cytoadherence level. Endothelial protein C receptor (EPCR)-binding domains were overexpres...Continue Reading

References

Jul 1, 1990·Statistics in Medicine·Y Hochberg, Y Benjamini
Aug 6, 1999·The American Journal of Pathology·K SilamutN J White
Feb 8, 2002·Nature·Louis H MillerOgobara K Doumbo
Jan 15, 2004·The Journal of Infectious Diseases·Sayeh JafariRémy Durand
Dec 15, 2007·Nature Protocols·Yuliang WuXing-Zhen Chen
Jun 3, 2010·Cold Spring Harbor Protocols·Donald C RioTimothy W Nilsen
Sep 24, 2010·PLoS Computational Biology·Thomas S RaskThomas Lavstsen
Jan 11, 2012·Journal of Visualized Experiments : JoVE·Antoine Claessens, J Alexandra Rowe
May 24, 2012·Proceedings of the National Academy of Sciences of the United States of America·Thomas LavstsenThor G Theander
May 24, 2012·Proceedings of the National Academy of Sciences of the United States of America·Marion AvrilJoseph D Smith
May 24, 2012·Proceedings of the National Academy of Sciences of the United States of America·Antoine ClaessensJ Alexandra Rowe
Mar 19, 2015·The New England Journal of Medicine·Karl B SeydelTerrie E Taylor
Sep 1, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Frank LennartzAnja T R Jensen
Mar 31, 2016·Journal of Visualized Experiments : JoVE·Mohameedyaseen SyedbashaAdrian Egli
Sep 3, 2017·The Journal of Infectious Diseases·Nicaise Tuikue NdamPhilippe Deloron
Jan 18, 2018·Proceedings of the National Academy of Sciences of the United States of America·Yvonne AzasiJ Alexandra Rowe
Jan 6, 2019·EMBO Molecular Medicine·Janet StormAlister G Craig
Feb 26, 2019·EMBO Molecular Medicine·Hannah Fleckenstein, Silvia Portugal
Jun 30, 2019·PloS One·Claire KamaliddinGwladys I Bertin
Feb 15, 2020·Wellcome Open Research·Thomas D OttoChris Newbold

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
ELISA
electrophoresis
Fusion Tribrid

Software Mentioned

Mascot

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.