PFG-NMR diffusometry: a tool for investigating the structure and dynamics of noncommercial purified pig gastric mucin in a wide range of concentrations

Biopolymers
G LafitteJ R Davies

Abstract

For the first time, Pulsed Field Gradient-Nuclear Magnetic Resonance, a powerful noninvasive tool for studying the dynamics and structure of complex gels, has been used to measure diffusion of probe molecules in aqueous solutions/gels of noncommercial purified pig gastric mucin (PGM), in a concentration range up to 5 wt %. Complementary data were obtained from rheology measurements. The combination of techniques revealed a strong pH dependency of the structure of the PGM samples while changes in concentration, ionic strength, and temperature appeared to induce less pronounced alterations. Viscosity was found to vary in a nonmonotonous way with pH, with the more viscous solutions found at intermediate pH. We propose that this finding is due to a reduced charge density at lower pH, which is expected to continuously increase the relative importance of hydrophobic associations. The results suggest a loose network of expanded fully charged PGM molecules with considerable mobility at neutral pH (pH 7.4). At intermediate pH (pH 4), a three-dimensional expanded network is favored. At pH 1, the charge density is low and microphase separation occurs since hydrophobic associations prevail. This leads to the formation of clusters concentra...Continue Reading

References

Jan 1, 1978·Biochemical Society Transactions·M Mantle, A Allen
Jan 1, 1992·Critical Reviews in Biochemistry and Molecular Biology·G J Strous, J Dekker
Jan 1, 1989·Advances in Carbohydrate Chemistry and Biochemistry·S E Harding
Mar 6, 1998·Pharmaceutical Research·A W LarhedE Björk
Feb 27, 1999·Biophysical Journal·X CaoNezam H Afdhal
Mar 4, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·Y HuangN A Peppas
Jun 7, 2002·Biophysical Chemistry·Robert TrampelKlaus Arnold
Apr 19, 2005·Magnetic Resonance Imaging·Roxane ColsenetFrançois Mariette
May 10, 2005·Biomacromolecules·Jonathan CelliShyamsunder Erramilli
Nov 15, 2005·Biomacromolecules·Zhenning HongNezam H Afdhal

Citations

Jan 15, 2014·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Marie BoeghHanne M Nielsen
Feb 2, 2013·The Journal of Physical Chemistry. B·Yana ZnamenskayaVitaly Kocherbitov
Jan 13, 2009·Advanced Drug Delivery Reviews·Yen Cu, W Mark Saltzman
Oct 23, 2008·Advanced Drug Delivery Reviews·Paola Occhipinti, Peter C Griffiths
Dec 1, 2007·Carbohydrate Research·Atoosa MalekiBo Nyström
Feb 3, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Julia GrießingerAndreas Bernkop-Schnürch
Jun 2, 2009·Advances in Colloid and Interface Science·Bo NyströmJosé García de la Torre
Nov 10, 2017·Advanced Healthcare Materials·Luise Tomasetti, Miriam Breunig

Related Concepts

Diffusion
Dose-Response Relationship, Drug
Gastric Mucins
In Vivo NMR Spectroscopy
Vigilon
Solutions
Structure-Activity Relationship
Thermodynamics
Deuterium Oxide

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Computational Methods for Protein Structures

Computational methods employing machine learning algorithms are powerful tools that can be used to predict the effect of mutations on protein structure. This is important in neurodegenerative disorders, where some mutations can cause the formation of toxic protein aggregations. This feed follows the latests insights into the relationships between mutation and protein structure leading to better understanding of disease.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.