pH- and ligand-induced release of loads from DNA-acrylamide hydrogel microcapsules

Chemical Science
Wei-Ching LiaoItamar Willner

Abstract

Herein, a method to construct stimuli-responsive DNA-acrylamide-based hydrogel microcapsules has been presented. This method involves the use of polyacrylamide chains modified with predesigned nucleic acid hairpin units and optionally single-strand tethers that provide the required hybridization and recognition functions to yield substrate-loaded stimuli-responsive hydrogel-based microcapsules. The synthesis of the microcapsules involves the loading of CaCO3 microparticles with the respective load substrates and the functionalization of the CaCO3 template particles with nucleic acid promoter units. In the presence of the hairpin-modified acrylamide chains, the promoter units induce the hybridization chain reaction (HCR), which leads to the formation of a hydrogel coating, which, after the dissociation of the CaCO3 cores, yields substrate-loaded stimuli-responsive hydrogel microcapsules. One of the microcapsule systems includes, in the hairpin-modified acrylamide constructs, and in the subsequent HCR-generated hydrogel shells, the caged sequences of anti-ATP or anti-cocaine aptamers. In the presence of ATP or cocaine, the duplex-caged aptamer sequences are separated via the formation of ATP- or cocaine-aptamer complexes, which r...Continue Reading

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Citations

Jan 3, 2019·Angewandte Chemie·Manish DebnathJyotirmayee Dash
Nov 16, 2019·Angewandte Chemie·Margarita Vázquez-González, Itamar Willner
Aug 16, 2017·Polymers·Javad Tavakoli, Youhong Tang
Oct 9, 2018·Current Medicinal Chemistry·Fen HeYanfei Liu
Nov 5, 2019·Frontiers in Chemistry·Dao-Yang FanZhong-Jun Liu
Feb 18, 2020·Topics in Current Chemistry·Fan XiaoLeilei Tian
Oct 20, 2020·Advanced Healthcare Materials·Fabio Rizzo, Nermin Seda Kehr
Nov 10, 2020·Advanced Materials·Alexandra E RangelH Tom Soh
Aug 25, 2020·Chemistry : a European Journal·Huimin WangXiaoqing Liu
Jul 28, 2020·Advanced Drug Delivery Reviews·Fangli MoWeihong Tan
Jan 17, 2021·Acta Biomaterialia·Elham SameiyanSeyed Mohammad Taghdisi
Jan 5, 2021·ACS Applied Materials & Interfaces·Margarita Vázquez-González, Itamar Willner
Mar 7, 2021·International Journal of Molecular Sciences·Margarita Vázquez-González, Itamar Willner
Aug 4, 2020·Advanced Drug Delivery Reviews·Nuria Oliva, Benjamin D Almquist
Aug 8, 2021·International Journal of Molecular Sciences·Luca AmbrosioVincenzo Denaro
Mar 30, 2018·ACS Applied Materials & Interfaces·Xia YangRuo Yuan
Mar 2, 2017·Accounts of Chemical Research·Jason S KahnItamar Willner
Jul 31, 2018·Macromolecules·Willem E M NotebornRoxanne E Kieltyka
Jun 6, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Margarita Vázquez-González, Itamar Willner

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Methods Mentioned

BETA
confocal microscopy
Fluorescence
flow cytometry
circular dichroism

Software Mentioned

Volocity
ImageJ

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