pH dependence of facilitation by neurotransmitters and divalent cations of P2X2 purinoceptor/channels

European Journal of Pharmacology
K NakazawaY Ohno

Abstract

The pH dependence of the facilitation by dopamine (10 microM), 5-hydroxytryptamine (10 microM), adenosine (1 and 100 microM), Zn2+ (10 microM) and Cd2+ (1 mM) of P2X2 purinoceptor/channels was tested by expressing these channels in Xenopus oocytes. In a pH range between 6.0 and 8.5, concentration-response curves for an inward current activated by ATP were shifted toward a lower concentration range at a more acidic pH, indicating that the sensitivity to ATP is pH-dependent. Comparison of the effects of the neurotransmitters and the divalent cations on the ATP-activated current was made using a concentration of ATP which activated 40-50% of the maximal current at each pH value. The current facilitation by dopamine was obvious at pH 7.1 and 7.7, but was not observed at pH 8.5. At pH 6.0, the current was inhibited upon first trials of dopamine, but it was facilitated upon second trials. With 5-hydroxytryptamine and adenosine, the current facilitation was most remarkable at pH 6.0, less remarkable at pH 7.1 and 7.7, and the facilitation was almost abolished at pH 8.5. On the other hand, the current facilitation by Zn2+ and Cd2+ was more remarkable at alkaline pH values (7.7 and 8.5), and the facilitation was almost abolished at pH 6...Continue Reading

References

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Citations

Oct 24, 2007·Molecular Neurobiology·Kerstin WirknerPeter Illes
Feb 27, 2003·Neuropharmacology·J D ClyneR I Hume
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·R A North, A Surprenant
Oct 31, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Rachel K Tittle, Richard I Hume
Aug 16, 2013·Antioxidants & Redox Signaling·Stanko S StojilkovicClaudio Coddou
Feb 1, 2005·European Journal of Pharmacology·Ken Nakazawa, Yasuo Ohno
Mar 8, 2002·The Journal of Physiology·J Dylan ClyneRichard I Hume
Sep 25, 2002·Physiological Reviews·R Alan North
Jan 19, 2021·Neuroscience·Rebecca F KrallElias Aizenman

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