pH-dependence of intermediate steps of membrane fusion induced by the influenza fusion peptide

The Biochemical Journal
Ding-Kwo Chang, Shu-Fang Cheng

Abstract

Membrane fusion mediated by the influenza-virus fusion protein is activated by low pH via a cascade of reactions. Some processes among them are irreversible, such as helix hairpin formation of the ectodomain, whereas others are reversible, such as exposure of the fusion peptide. Using this property, we attempted to dissect, in temporal order, different stages of the fusion reaction involving the fusion peptide by an acidic-neutral-acidic pH cycle. The fluorescence-quenching data indicated that both insertion depth and self-assembly are pH-reversible. In addition, lipid mixing assay was demonstrated to be arrested by neutral pH. By contrast, membrane leakage was shown to be irreversible with respect to pH. Our results, along with those from other studies on the pH-dependence of membrane fusion, are used to build a model for the virus-mediated fusion event from the perspective of pH-reversibility.

References

Aug 1, 1988·The Journal of General Virology·S A WhartonD C Wiley
Apr 1, 1986·Biochemical Society Transactions·J J SkehelD C Willey
Oct 15, 1994·Structure·D Baker, D A Agard
Mar 23, 1993·Biochemistry·J Ramalho-SantosM da C de Lima
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·J ChenD C Wiley
Jul 19, 1996·Journal of Molecular Biology·S A Tatulian, L K Tamm
Dec 1, 1995·Nature Structural Biology·M LuP S Kim
Nov 25, 1998·Protein Science : a Publication of the Protein Society·C Gray, L K Tamm
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·J ChenD C Wiley
Aug 31, 2000·Annual Review of Biochemistry·J J Skehel, D C Wiley
Apr 5, 2002·Journal of Virology·Jennifer A GruenkeJudith M White
Nov 2, 2002·The EMBO Journal·Eugenia LeikinaLeonid V Chernomordik
Mar 13, 2003·Molecular Biology of the Cell·Ruben M MarkosyanGrigory B Melikyan
Oct 12, 2004·Biophysical Journal·Loredana VaccaroFranca Fraternali

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.