pH dependence of salt activation of human leukocyte elastase

Archives of Biochemistry and Biophysics
G OshimaM Yamada


The effects of pH on salt stimulation of the rates of hydrolysis of three substrates by human leukocyte elastase were studied. The enzyme was most active at pH 10.5, 8.0-8.5, and 9.5 for the hydrolyses of fluorescein isothiocyanate-labeled S-carboxymethylated bovine serum albumin (FITC-CM-BSA), succinyl-L-Ala-L-Pro-L-Ala-7-methylcoumaryl-4-amide (Suc-APA-MCA), and succinyl-L-Ala3-p-nitroanilide (Suc-Ala3-pNA), respectively, in the absence of NaCl. The enzyme was activated by 0.5 M NaCl similarly at all pHs tested for the hydrolysis of Suc-Ala3-pNA, but more at neutral and alkaline pH values, respectively, for the hydrolyses of FITC-CM-BSA and Suc-APA-MCA. Thus, in the presence of 0.5 M NaCl, the enzyme was most active at pH 8.0 and 10.0 with FITC-CM-BSA and Suc-APA-MCA, respectively. In contrast, the proteolytic activity of porcine pancreatic elastase was somewhat inhibited by 0.5 M NaCl.


May 1, 1976·The Biochemical Journal·P M Starkey, A J Barrett
Nov 1, 1978·European Journal of Immunology·A SolomonK Havemann
May 11, 1978·Biochimica Et Biophysica Acta·A R BakkenistB F van Gelder
Feb 27, 1975·Biochimica Et Biophysica Acta·R Rindler-Ludwig, H Braunsteiner
Sep 12, 1979·Biochemical and Biophysical Research Communications·G OshimaT Sugimura
May 1, 1976·The Biochemical Journal·P M Starkey, A J Barrett
Oct 1, 1976·European Journal of Biochemistry·M Delshammar, K Ohlsson
Feb 24, 1976·Biochemistry·R J Baugh, J Travis
Sep 1, 1974·Hoppe-Seyler's Zeitschrift für physiologische Chemie·W Schmidt, K Havemann

❮ Previous
Next ❯


Feb 1, 1992·Biochimica Et Biophysica Acta·T Imamura, T Kambara
Jul 7, 2007·Scandinavian Journal of Gastroenterology·Tadashi KohnoKunihiko Shibata
Dec 28, 1999·Respirology : Official Journal of the Asian Pacific Society of Respirology·W IchikawaS Sone
Oct 14, 2018·The Journal of Biological Chemistry·Takushi FujimotoHiroshi Kadokura
Aug 1, 1990·Biological Chemistry Hoppe-Seyler·H R WenzelH Tschesche
Oct 1, 1991·Biological Chemistry Hoppe-Seyler·G Oshima, M Yamada
Sep 23, 1997·Biochemical and Biophysical Research Communications·K SudoT Yokota
May 15, 1990·Thrombosis Research·G Oshima

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.