Aug 10, 1976

pH dependence of tritium exchange with the C-2 protons of the histidines in bovine trypsin

M KriegerR M Stroud


At pH 8.9 and 37 degrees C the half-times for tritium exchange with the C-2 protons of the histidines of trypsin are 73 days for His-57, and greater than 1000 days for His-40 and His-91. These half-times are much longer than the half-life of exchange for the C-2 proton of free histidine (2.8 days at pD 8.2), and longer than any previously reported half-time of exchange at pH greater than 8. These very low rates of exchange are discussed with reference to the refined structure of trypsin. The tritium exchange of His-57 depends on an apparent pKa of 6.6. This pKa may represent the pKa of the imidazole of His-57 in an inactive conformation of the enzyme.

Mentioned in this Paper

Trypsin Inhibitor, Kunitz Soybean
Isotope Labeling, Stable
Plasma Protein Binding Capacity
Peptide Fragments
Bos indicus
Hydrogen-Ion Concentration

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