PMID: 3754765Apr 22, 1986Paper

pH-dependent lysis of liposomes by adenovirus

Biochemistry
R BlumenthalI H Pastan

Abstract

Purified adenovirus induced a dose-dependent release of the water-soluble markers calcein and carboxyfluorescein from liposomes. Marker release was strongly dependent on pH, and at temperatures below 5 degrees C, the rate of release showed an optimum at a pH of about 6. This pH dependence parallels disruption of endocytic vesicles by adenovirus and the permeabilization that adenovirus induces on the cell surface. There did not seem to be a striking dependence on the lipid composition of the liposomes. Electron microscopy using a negative stain shows liposomes bound to adenovirus. In some cases, the liposomes were still intact, but many liposomes, which were attached to the vertices of the virus, appeared lysed. These data support the notion that adenovirus, which enters the host cell by receptor-mediated endocytosis, gains access to the cytoplasm by a subsequent pH-dependent disruption of the membrane of the endocytic vesicle.

Citations

Oct 15, 2009·Amino Acids·Manjula Kalia, Shahid Jameel
Jun 18, 1991·Biochimica Et Biophysica Acta·R B SpruijtM A Hemminga
Oct 26, 1995·Journal of Immunological Methods·J R MerwinH C Chiou
Jan 1, 1989·Pharmacology & Therapeutics·L CarrascoJ L Castrillo
Dec 1, 1993·Current Opinion in Biotechnology·M Cotten, E Wagner
Mar 1, 1995·Biologicals : Journal of the International Association of Biological Standardization·S C Phillips
Apr 7, 2004·Advanced Drug Delivery Reviews·David Sheff
Jun 6, 2000·Advanced Drug Delivery Reviews· Shapiro Ledley T, F D Ledley
Aug 14, 2003·Trends in Microbiology·Kartik Chandran, Max L Nibert
Jul 31, 2013·Proceedings of the National Academy of Sciences of the United States of America·Brandon B HolmesMarc I Diamond
Oct 1, 1994·The Journal of Cell Biology·T J WickhamG R Nemerow
Oct 31, 2012·Current Opinion in Neurology·Brandon B Holmes, Marc I Diamond
Apr 5, 2002·Journal of Virology·Sanna SuikkanenMatti Vuento
Mar 29, 2003·Journal of Virology·Larissa BalakirevaJadwiga Chroboczek
Feb 1, 2005·Journal of Virology·Christopher M WiethoffGlen R Nemerow
May 23, 2009·PLoS Pathogens·Sara SalinasEric J Kremer
Jul 30, 1997·Biochemical and Biophysical Research Communications·A A RosenkranzA S Sobolev
Jan 6, 2010·EMBO Molecular Medicine·Katrin Tiemann, John J Rossi
Jun 21, 2011·Biochimica Et Biophysica Acta·Sarah E StewartPhillip I Bird
Sep 13, 2013·Journal of Virology·Maarit SuomalainenUrs F Greber
Dec 24, 2005·Journal of Pharmaceutical Sciences·Jason RexroadC Russell Middaugh
Jul 20, 2010·Current Opinion in Biotechnology·V Ann Chailertvanitkul, Colin W Pouton
Jul 5, 2003·The Journal of Pharmacy and Pharmacology·Yong Woo ChoKinam Park
Jun 11, 2003·The Journal of Gene Medicine·Oliver Meier, Urs F Greber
Jul 16, 2010·Reviews in Medical Virology·Renate Fuchs, Dieter Blaas
Aug 7, 2004·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·S DrotleffA Göpferich
Aug 10, 2004·Biophysical Journal·Marisa Martin-FernandezGareth R Jones
Jun 28, 2005·Biophysical Journal·Anh-Tuan DinhSamir Mitragotri
May 11, 2005·Biochemical and Biophysical Research Communications·Hideyo UgaiKazunari K Yokoyama
Mar 3, 2015·Virology·Samuel Kilcher, Jason Mercer
Jan 5, 2005·Molecular Cell·Chloe ZubietaStephen Cusack
Oct 26, 2013·Advances in Biological Regulation·Kenza Snoussi, Michael Kann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.