PMID: 19961414Dec 8, 2009

pH-Dependent urea-induced unfolding of stem bromelain: unusual stability against urea at neutral pH

Biochemistry. Biokhimii︠a︡
Basir AhmadRizwan H Khan

Abstract

Equilibrium unfolding of stem bromelain (SB) with urea as a denaturant has been monitored as a function of pH using circular dichroism and fluorescence emission spectroscopy. Urea-induced denaturation studies at pH 4.5 showed that SB unfolds through a two-state mechanism and yields DeltaG (free energy difference between the fully folded and unfolded forms) of approximately 5.0 kcal/mol and C(m) (midpoint of the unfolding transition) of approximately 6.5 M at 25 degrees C. Very high concentration of urea (9.5 M) provides unusual stability to the protein with no more structural loss and transition to a completely unfolded state.

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Related Concepts

Stem bromelain
Dayto Anase
Circular Dichroism, Vibrational
Hydrogen-Ion Concentration
Protein Denaturation
Fluorescence Spectroscopy
Thermodynamics
Carmol
Stem Cells
Fluorescence Spectroscopy

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