pH Effect and Chemical Mechanisms of Antioxidant Higenamine

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Yulu XieDongfeng Chen

Abstract

In this article, we determine the pH effect and chemical mechanism of antioxidant higenamine by using four spectrophotometric assays: (1) 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide radical (PTIO•)-scavenging assay (at pH 4.5, 6.0, and 7.4); (2) Fe3+-reducing power assay; (3) Cu2+-reducing power assay; and (4) 1,1-diphenyl-2-picryl-hydrazyl (DPPH•)-scavenging assay. The DPPH•-scavenging reaction product is further analyzed by ultra-performance liquid chromatography, coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-Q-TOF-MS/MS) technology. In the four spectrophotometric assays, higenamine showed good dose-response curves; however, its IC50 values were always lower than those of Trolox. In UPLC-ESI-Q-TOF-MS/MS analysis, the higenamine reaction product with DPPH• displayed three chromatographic peaks (retention time = 0.969, 1.078, and 1.319 min). The first gave m/z 541.2324 and 542.2372 MS peaks; while the last two generated two similar MS peaks (m/z 663.1580 and 664.1885), and two MS/MS peaks (m/z 195.9997 and 225.9971). In the PTIO•-scavenging assays, higenamine greatly decreased its IC50 values with increasing pH. In conclusion, higenamine is a powerful antioxidant-it...Continue Reading

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Sep 23, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xican LiDongfeng Chen
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Jul 15, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xican LiDongfeng Chen
Oct 2, 2017·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Yunning YangXing Tang
Jan 25, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yage TianDongfeng Chen
Jan 25, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xican LiDongfeng Chen
Jan 25, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hong XieDongfeng Chen
Apr 25, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xican LiDongfeng Chen
May 15, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xican LiDongfeng Chen

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Citations

Nov 30, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiaojian OuyangDongfeng Chen
Dec 24, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Minshi LiangDongfeng Chen
Aug 2, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xican LiDongfeng Chen
Sep 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jihua XuYugang Zhang
Feb 9, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jie LiuDongfeng Chen
Apr 30, 2020·Antioxidants·Isabella RomeoTiziana Marino
Nov 17, 2020·International Journal of Veterinary Science and Medicine·Sugiharto SugihartoTri Agus Sartono
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ban ChenDongfeng Chen
Jul 25, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lin YangXican Li

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