pH-induced conformation changes of adsorbed vitronectin maximize its bovine aortic endothelial cell binding ability

Journal of Biomedical Materials Research. Part a
Matthew T Bernards, Shaoyi Jiang

Abstract

Vitronectin (VN) is an important matricellular protein that plays a role in cell adhesion, cell migration, wound healing, and inflammation. VN is present in both serum and plasma and studies have shown that it has significant surface enrichment on implanted biomaterials as compared to other proteins present in plasma. Previously, charged self-assembled monolayers (SAMs) have been shown to influence the orientation and conformation of adsorbed proteins and their subsequent bioactivity. In addition, numerous studies have shown that the conformation of VN plays a critical role in its bioactive properties. In this study, both the orientation and conformation of adsorbed VN are systematically studied to determine which of these has the greater influence on its cell binding ability. Atomic force microscopy was used to qualitatively asses the adsorbed amount of VN on positively and negatively charged SAMs both before and after conformation changes induced by pH. Cell adhesion assays were then performed to compare the bovine aortic endothelial cell (BAEC) binding ability of VN in three different conformations on positively and negatively charged surfaces. The results indicate that conformation, not orientation, plays the more important...Continue Reading

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Citations

Apr 19, 2008·Colloids and Surfaces. B, Biointerfaces·Matthew T BernardsShaoyi Jiang
Aug 26, 2009·Journal of the European Academy of Dermatology and Venereology : JEADV·S SchremlP Babilas
Apr 11, 2015·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Carla R KruseJens A Sørensen
Feb 9, 2013·Acta Biomaterialia·Helka JuvonenJouko Peltonen
Nov 6, 2012·Journal of Zhejiang University. Science. B·Paweł OlczykKrystyna Olczyk
Dec 21, 2016·Macromolecular Bioscience·Julia KoehlerFerdinand P Brandl
Jul 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Deepthi SankarJayakumar Rangasamy

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