pH-Mediated Microbial and Metabolic Interactions in Fecal Enrichment Cultures

MSphere
Zehra Esra IlhanRosa Krajmalnik-Brown

Abstract

pH and fermentable substrates impose selective pressures on gut microbial communities and their metabolisms. We evaluated the relative contributions of pH, alkalinity, and substrate on microbial community structure, metabolism, and functional interactions using triplicate batch cultures started from fecal slurry and incubated with an initial pH of 6.0, 6.5, or 6.9 and 10 mM glucose, fructose, or cellobiose as the carbon substrate. We analyzed 16S rRNA gene sequences and fermentation products. Microbial diversity was driven by both pH and substrate type. Due to insufficient alkalinity, a drop in pH from 6.0 to ~4.5 clustered pH 6.0 cultures together and distant from pH 6.5 and 6.9 cultures, which experienced only small pH drops. Cellobiose yielded more acidity than alkalinity due to the amount of fermentable carbon, which moved cellobiose pH 6.5 cultures away from other pH 6.5 cultures. The impact of pH on microbial community structure was reflected by fermentative metabolism. Lactate accumulation occurred in pH 6.0 cultures, whereas propionate and acetate accumulations were observed in pH 6.5 and 6.9 cultures and independently from the type of substrate provided. Finally, pH had an impact on the interactions between lactate-pro...Continue Reading

References

Jan 1, 1992·The Journal of Applied Bacteriology·G T MacfarlaneJ H Cummings
Jun 1, 1991·The Journal of Applied Bacteriology·J H Cummings, G T Macfarlane
Sep 1, 1989·FEMS Microbiology Reviews·D P Clark
Aug 1, 1986·Infection and Immunity·G R BenderR E Marquis
Sep 1, 1981·Applied and Environmental Microbiology·T L Miller, M J Wolin
Jun 3, 1982·The New England Journal of Medicine·L StolbergS Finegold
Feb 1, 1993·Critical Care Medicine·R G Fiddian-Green
Nov 1, 1995·Critical Reviews in Food Science and Nutrition·J van LooG Smits
Sep 24, 1998·Caries Research·D J Bradshaw, P D Marsh
Oct 26, 1999·Journal of Gastroenterology and Hepatology·S PatraM M Mathan
Aug 30, 2002·Applied and Environmental Microbiology·Dora I A Pereira, Glenn R Gibson
Oct 7, 2004·Applied and Environmental Microbiology·Sylvia H DuncanHarry J Flint
Feb 9, 2005·The American Journal of Clinical Nutrition·Loren CordainJanette Brand-Miller
Feb 11, 2005·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·S DavailR Hoo-Paris
Jul 8, 2005·Journal of Clinical Microbiology·Alexander SwidsinskiHerbert Lochs
Sep 27, 2005·Journal of the American Dietetic Association·Peter L BeyerRichard W McCallum
Jun 1, 1979·Applied and Environmental Microbiology·J E Schultz, J A Breznak
Apr 1, 1995·Applied and Environmental Microbiology·A RamosH Santos
Jul 6, 2006·Applied and Environmental Microbiology·T Z DeSantisG L Andersen
Jun 26, 2007·Applied and Environmental Microbiology·Qiong WangJames R Cole
Sep 4, 2007·Applied and Environmental Microbiology·Alvaro BelenguerHarry J Flint
May 29, 2008·Environmental Science & Technology·Hyung-Sool LeeBruce E Rittmann
Jan 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Husen ZhangRosa Krajmalnik-Brown
Apr 29, 2009·Environmental Microbiology·Sylvia H DuncanHarry J Flint
Nov 17, 2009·Bioinformatics·J Gregory CaporasoRob Knight
Jan 21, 2010·Physiological Reviews·Luc Tappy, Kim-Anne Lê
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
May 15, 2010·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·M CamilleriS S C Rao
Aug 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·Carlotta De FilippoPaolo Lionetti
Aug 17, 2010·Bioinformatics·Robert C Edgar

❮ Previous
Next ❯

Citations

Jan 16, 2019·Journal of Bacteriology·Tara GallagherKatrine Whiteson
Mar 19, 2019·Experimental Biology and Medicine·Anders B Dohlman, Xiling Shen
Aug 7, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Claudio BernardazziFayez K Ghishan
Jan 5, 2020·Nutritional Neuroscience·John C PerhamJulia J Rucklidge
Oct 13, 2020·FEMS Microbiology Ecology·Ana Margarida PereiraAna Rita J Cabrita
Feb 5, 2021·IScience·Jocelyn M Choo, Geraint B Rogers
Mar 5, 2021·Scientific Reports·Núria MachAlice Ruet
May 25, 2021·Pflügers Archiv : European journal of physiology·Friederike StumpffHolger Martens
Jun 2, 2021·Nature Communications·Ryan L ClarkOphelia S Venturelli
Jun 3, 2021·International Journal of Molecular Sciences·David ManneckFriederike Stumpff
Sep 24, 2021·The Proceedings of the Nutrition Society·Catriona ThomsonChristine A Edwards
Dec 2, 2021·Proceedings. Biological Sciences·Evan C Palmer-YoungJay D Evans

❮ Previous
Next ❯

Methods Mentioned

BETA
gastric bypass
PCR

Software Mentioned

PyNAST
UniFrac
Uclust
Greengenes
QIIME
ACE
Statistical Package for Social Sciences
ChimeraSlayer

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.