pH-Regulated Synthesis of Trypsin-Templated Copper Nanoclusters with Blue and Yellow Fluorescent Emission

ACS Omega
Jie FengXingguo Chen

Abstract

In this article, a simple protocol to prepare water-soluble fluorescent copper nanoclusters (CuNCs) using trypsin as a stabilizer and hydrazine hydrate as a reducing agent was reported. It was found that the pH of the reaction solution was critical in determining the fluorescence of CuNCs. CuNCs with blue and yellow fluorescent emission were obtained under basic and acidic conditions, respectively. Although the detailed formation mechanisms of these CuNCs required further analysis, the synthetic route was promising for preparing different fluorescent metal NCs for applications. With good water solubility and excellent photostability, the yellow-emitting CuNCs could serve as a fluorescence probe for detection of Hg2+ based on the aggregation-induced quenching mechanism. The fluorescence quenching efficiency had fantastic linearity to Hg2+ concentrations in the range of 0.1-100 μM, with a limit of detection of 30 nM. Additionally, the yellow-emitting CuNCs exhibited negligible cytotoxicity and were successfully applied to bioimaging of HeLa cells.

References

Aug 15, 2002·Analytical Chemistry·Xiaohe XuHai-Feng Ji
Jul 28, 2006·Organic Letters·Ki Cheol SongSuk-Kyu Chang
Apr 5, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Derrick MottChuan-Jian Zhong
Aug 19, 2007·Biochimica Et Biophysica Acta·Andreas Barth
Jan 7, 2009·Journal of the American Chemical Society·Jianping XieJackie Y Ying
Feb 2, 2011·Journal of the American Chemical Society·Wentao WeiShaowei Chen
Mar 24, 2011·Analytical Chemistry·Cunlan Guo, Joseph Irudayaraj
Apr 19, 2011·Analytical Chemistry·Dan WenShaojun Dong
Jun 15, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hideya KawasakiRyuichi Arakawa
Sep 7, 2011·Chemical Communications : Chem Comm·Liu DengShaojun Dong
Oct 21, 2011·ACS Nano·Kamalesh ChaudhariThalappil Pradeep
Nov 5, 2011·Analytical Chemistry·Nirmal GoswamiSamir Kumar Pal
Mar 9, 2012·Organic Letters·Mari IkedaYoichi Habata
Apr 25, 2012·Journal of the American Chemical Society·Kristie J KoskiYi Cui
May 15, 2013·Small·Xiaofang JiaErkang Wang
Nov 23, 2013·Chemical Communications : Chem Comm·Zhentao LuoJianping Xie
Jan 22, 2014·ACS Applied Materials & Interfaces·V VenkateshSandeep Verma
Feb 18, 2014·Analytical Chemistry·Subhadip GhoshSaptarshi Mukherjee
Feb 28, 2014·ACS Applied Materials & Interfaces·Rama GhoshArun Chattopadhyay
Oct 18, 2014·ACS Applied Materials & Interfaces·Heng-Chia ChangJa-An Annie Ho

❮ Previous
Next ❯

Citations

Mar 16, 2019·Angewandte Chemie·Antonio AiresAitziber L Cortajarena
Nov 18, 2018·Biosensors·Antonio AiresAitziber L Cortajarena

❮ Previous
Next ❯

Methods Mentioned

BETA
transmission electron microscopy
surface plasmon resonance
circular dichroism
X-ray
Fluorescence

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.