pH Regulation of ammonia secretion by Colletotrichum gloeosporioides and its effect on appressorium formation and pathogenicity

Molecular Plant-microbe Interactions : MPMI
I MiyaraD Prusky

Abstract

Host-tissue alkalinization via ammonia accumulation is key to Colletotrichum spp. colonization. Using macroarrays carrying C. gloeosporioides cDNAs, we monitored gene expression during the alkalinization process. A set of genes involved in synthesis and catabolism of ammonia accumulation were identified. Expression of NAD(+)-specific glutamate dehydrogenase (GDH2, encoding ammonia synthesis) and the ammonia exporter AMET were induced at pH 4.0 to 4.5. Conversely, ammonia uptake and transcript activation of the ammonia and glutamate importers (MEP and GLT, respectively) and glutamine synthase (GS1) were higher at pH 6.0 to 7.0. Accumulated ammonia in the wild-type mycelium decreased during ambient alkalinization, concurrent with increased GS1 expression. Deltapac1 mutants of C. gloeosporioides, which are sensitive to alkaline pH changes, showed upregulation of the acid-expressed GDH2 and downregulation of the alkaline-expressed GS1, resulting in 60% higher ammonia accumulation inside the mycelium. Deltagdh2 strains of C. gloeosporioides, impaired in ammonia production, showed 85% inhibition in appressorium formation followed by reduced colonization on avocado fruit (Persea americana cv. Fuerte) pericarp, while exogenic ammonia a...Continue Reading

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Citations

Aug 7, 2013·Applied Microbiology and Biotechnology·Tianyuan ZhangHongye Li
Dec 20, 2012·Antioxidants & Redox Signaling·Noam AlkanDov Prusky
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Jul 9, 2021·Ecotoxicology and Environmental Safety·Shasha XiaoYan Wang

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