pH-responsive high stability polymeric nanoparticles for targeted delivery of anticancer therapeutics.

Communications Biology
L PalanikumarMazin Magzoub

Abstract

The practical application of nanoparticles (NPs) as chemotherapeutic drug delivery systems is often hampered by issues such as poor circulation stability and targeting inefficiency. Here, we have utilized a simple approach to prepare biocompatible and biodegradable pH-responsive hybrid NPs that overcome these issues. The NPs consist of a drug-loaded polylactic-co-glycolic acid (PLGA) core covalently 'wrapped' with a crosslinked bovine serum albumin (BSA) shell designed to minimize interactions with serum proteins and macrophages that inhibit target recognition. The shell is functionalized with the acidity-triggered rational membrane (ATRAM) peptide to facilitate internalization specifically into cancer cells within the acidic tumor microenvironment. Following uptake, the unique intracellular conditions of cancer cells degrade the NPs, thereby releasing the chemotherapeutic cargo. The drug-loaded NPs showed potent anticancer activity in vitro and in vivo while exhibiting no toxicity to healthy tissue. Our results demonstrate that the ATRAM-BSA-PLGA NPs are a promising targeted cancer drug delivery platform.

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Citations

Apr 25, 2020·Polymers·Tanja NarancicKevin E O'Connor
Aug 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Aleksandra ZielińskaEliana B Souto
Jan 29, 2021·International Journal of Biological Macromolecules·Rakesh Kumar MishraRehan Khan
Jan 30, 2021·Bioresource Technology·Pawankumar RaiSandeep K Sharma
Feb 4, 2021·Biomedicines·Sulltana JahanEzharul Hoque Chowdhury
Dec 6, 2020·Nature Reviews. Drug Discovery·Michael J MitchellRobert Langer
Jan 5, 2021·Frontiers in Molecular Biosciences·Reju George ThomasYong Yeon Jeong
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Apr 4, 2021·Pharmaceutics·Valentina Di FrancescoMiguel Ferreira
Jul 31, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Pragya PrasannaDebabrata Mandal
Jul 28, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Asmita BanstolaSimmyung Yook

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Methods Mentioned

BETA
transmission electron microscopy
infrared spectroscopy
protein assay
scanning
circular dichroism
FRET
fluorescence microscopy
flow cytometry
confocal microscopy
Flow

Software Mentioned

Mascot
FACSDiva
Prism
BD
DataAnalysis
ProteinScape
MaxQuant
GraphPad
Andromeda
Fiji

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