pH-sensitive and pluronic-modified pullulan nanogels for greatly improved antitumor in vivo

International Journal of Biological Macromolecules
Yan ZhengRupei Tang

Abstract

It remains a crucial challenge to achieve efficient cellular uptake in tumor cells for nanoscale drug delivery systems. This work described that two multi-functional pullulan nanogels were prepared by co-polymerization between methacrylated pullulan (Pullulan-M) and different crosslink agents, an acid-labile ortho ester-modified pluronic (L61-MOE) or non-acid-sensitive methacrylated pluronic (L61-M). The prepared nanogels showed a regular spherical structure with the size about 200 nm measured by dynamic light scattering and transmission electron microscopy (TEM). Doxorubicin as a model drug was successfully encapsulated into nanogels. As expected, Pul-L61-MOE showed pH-dependent DOX release, and 25% of DOX was released at pH 7.4 while 84.48% of DOX was released at pH 5.0. In vitro cellular uptake and MTT results indicated that pH-sensitive nanogels (Pul-L61-MOE) displayed higher cellular internalization and cytotoxicity than acid-insensitive nanogels (Pul-L61-M) and free DOX. Flow cytometry assay suggested these nanogels remarkably increased intracellular reactive oxygen species (ROS) level and induced more cell apoptosis by the function of pluronic. Finally, in vivo antitumor results indicated that the multi-functional nanoge...Continue Reading

Citations

Jun 26, 2020·International Journal of Nanomedicine·Jianping DengZhiping Wang
Oct 27, 2020·Nanomedicine·Filippo PinelliFilippo Rossi
Nov 3, 2020·International Journal of Biological Macromolecules·Ram Sarup SinghJohn F Kennedy
May 2, 2021·International Journal of Biological Macromolecules·Lei LiuShiyong Song
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jialin YuYang Li
Aug 9, 2021·International Journal of Biological Macromolecules·Xu ChengRupei Tang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis