PMID: 8584413Nov 1, 1995Paper

pH sensitivity of the cardiac gap junction proteins, connexin 45 and 43

Pflügers Archiv : European journal of physiology
M M HermansM B Rook

Abstract

. Intercellular communication through gap junction channels can be regulated by changes in intracellular pH (pHi). This regulation may play an important role in ischemic heart tissue. Using the dual voltage-clamp technique, we compared the pHi sensitivity of gap junction channels composed of connexin 43 (Cx43) and Cx45, two of the gap junction proteins that are expressed in heart. We made use of SKHep1 cells, endogenously expressing low levels of Cx45 and SKHep1 cells stably transfected with rat Cx43. To manipulate the pHi we applied the NH3/NH+4 pH-clamp method. At pHi 6.7 the gj of Cx45 channels was reduced to approximately 20% of control values (pHi 7.0) and at pHi 6.3 all channels closed. The gj of Cx43 channels was approximately 70% of control values at pHi 6.7 and approximately 40% at pHi 6.3. Cx43 channels closed at pHi 5.8. Single channel conductances were 17.8 pS for Cx45 and 40.8 pS for Cx43 at pHi 7.0 and did not change significantly at lower pHi. This suggests that the decrease in macroscopic conductance observed at low pHi results from the decrease in open probability of gap junctional channels rather than from a decrease in single channel conductance. Our results demonstrate that gap junction channels built of Cx4...Continue Reading

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Citations

Apr 1, 2004·BMC Medical Informatics and Decision Making·Jean-Charles DufourMarius Fieschi
Mar 25, 2009·BMC Medical Informatics and Decision Making·Kensaku KawamotoGeoffrey S Ginsburg
Jan 6, 2010·BMC Health Services Research·Gianfranco DamianiWalter Ricciardi
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