pH-Triggered Adhesiveness and Cohesiveness of Chondroitin Sulfate-Catechol Biopolymer for Biomedical Applications

Frontiers in Bioengineering and Biotechnology
Annachiara ScalzonePiergiorgio Gentile

Abstract

Nature provides biomaterials that tend to be effective to control both their adhesive and cohesive properties. A catecholamine motif found in the marine mussels, the mytilus edulis foot protein, can play adhesiveness and cohesiveness. Particularly, acidic pH drives catechol (Cat) to have adhesive function, resulting in surface coating, while basic pH allows to enhance its cohesive properties, resulting in the formation of hydrogels. In this work, we demonstrated the usefulness of Cat-conjugated chondroitin sulfate (CS) as a platform for mesenchymal stem cell culture, utilizing the adhesive property of CS-Cat as coating for different substrates and the cohesive properties as hydrogel for cells encapsulation. To prepare the CS-Cat biopolymer, dopamine (DP) was coupled to the CS by carbodiimide coupling reaction and the Cat content was determined by UV-Vis spectroscopy (4.8 ± 0.6%). To demonstrate the adhesive properties of the biopolymer, PLA, PCL, TiO2, and SiO2 substrates were immersed in CS-Cat solution (pH < 2). Following the coating, the surfaces became highly hydrophilic, exhibiting a contact angle less than 35°. Also, in the presence of an oxidizing agent at pH 8, CS-Cat solution immediately became a hydrogel, as shown by ...Continue Reading

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Citations

Mar 13, 2021·Biomaterials Science·Kresanti D NgadiminAna M Ferreira

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Methods Mentioned

BETA
NMR
X-ray
scanning electron microscopy
infrared spectroscopy
bioprinting

Software Mentioned

Drop Shape Analysis System DSA
ImageJ
GraphPad Prism

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