PMID: 9423843Jan 10, 1998Paper

Phage antibodies obtained by competitive selection on complement-resistant Moraxella (Branhamella) catarrhalis recognize the high-molecular-weight outer membrane protein

Infection and Immunity
E BoelT Logtenberg

Abstract

We used competitive panning to select a panel of 10 different human antibodies from a large semisynthetic phage display library that distinguish between serum complement-resistant and complement-sensitive strains of the gram-negative diplococcus Moraxella (Branhamella) catarrhalis. Western blotting analyses and inhibition enzyme-linked immunosorbent assays showed that all phage antibodies were directed against the same or closely spaced epitopes on the target protein, which is the high-molecular-weight outer membrane protein (HMW-OMP) of M. catarrhalis. HMW-OMP was found in multiple isolates of complement-resistant but not complement-sensitive M. catarrhalis strains. Nucleotide sequence analysis demonstrated that the immunoglobulin heavy- and light-chain variable-region genes encoding the 10 phage antibodies were remarkably similar, with a strong preference for basic amino acid residues in the heavy-chain CDR3 regions. This is the first report showing that competitive panning is a successful procedure to obtain phage antibodies against differentially expressed structures on phenotypically dissimilar strains of prokaryotic cells.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 1, 1992·The American Review of Respiratory Disease·T F Murphy, S Sethi
May 1, 1991·Journal of Bacteriology·R E MandrellR Yamasaki
Dec 5, 1991·Journal of Molecular Biology·J D MarksG Winter
May 6, 1991·The Medical Journal of Australia·F M BoyleJ G McCormack
Oct 1, 1990·Clinical Microbiology Reviews·B W Catlin
Jul 1, 1989·The Journal of Infectious Diseases·P BrandtzaegP Kierulf
Jan 1, 1989·Methods in Enzymology·A Plückthun, A Skerra
Nov 1, 1987·Reviews of Infectious Diseases·H HagerS L Berk
Oct 1, 1988·The Journal of Infectious Diseases·L C Bartos, T F Murphy
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·J de KruifT Logtenberg
Jun 1, 1995·FEMS Immunology and Medical Microbiology·C HolH van Dijk
May 1, 1995·Clinical and Diagnostic Laboratory Immunology·C M VerduinH Van Dijk
Jan 1, 1993·Trends in Biotechnology·G GeorgiouJ A Francisco
Jan 1, 1994·Advances in Immunology·D R Burton, C F Barbas
Jan 1, 1994·Annual Review of Immunology·G WinterH R Hoogenboom
Oct 1, 1996·Immunology Today·J de KruifT Logtenberg

❮ Previous
Next ❯

Citations

Aug 17, 2004·Clinical Chemistry·Jennifer BrigatiValery A Petrenko
Sep 1, 1999·Immunology·A van der Vuurst de Vries, T Logtenberg
May 21, 2016·Proteomics. Clinical Applications·Philipp KuhnMichael Hust
Sep 2, 2009·Microbiology and Molecular Biology Reviews : MMBR·Stefan P W de VriesPeter W M Hermans
May 10, 2003·Journal of Immunological Methods·Andrew HayhurstGeorge Georgiou
Jul 6, 2004·Journal of Microbiological Methods·Valery A Petrenko, Iryna B Sorokulova
Jul 27, 2021·Frontiers in Cellular and Infection Microbiology·Kristian Daniel Ralph RothMichael Hust
Sep 27, 2007·Genome Research·Thierry WirthMark Achtman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.